插入序列 ISRP10 失活耐亚胺培南铜绿假单胞菌临床分离株中的 oprD 基因。
Insertion sequence ISRP10 inactivation of the oprD gene in imipenem-resistant Pseudomonas aeruginosa clinical isolates.
机构信息
Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Tianjin Children's Hospital, Tianjin 300074, China.
出版信息
Int J Antimicrob Agents. 2016 May;47(5):375-9. doi: 10.1016/j.ijantimicag.2016.02.008. Epub 2016 Mar 24.
Carbapenem resistance mechanisms were investigated in 32 imipenem-resistant Pseudomonas aeruginosa clinical isolates recovered from hospitalised children. Sequence analysis revealed that 31 of the isolates had an insertion sequence element ISRP10 disrupting the porin gene oprD, demonstrating that ISRP10 inactivation of oprD conferred imipenem resistance in the majority of the isolates. Multilocus sequence typing (MLST) was used to discriminate the isolates. In total, 11 sequence types (STs) were identified including 3 novel STs, and 68.3% (28/41) of the tested strains were characterised as clone ST253. In combination with random amplified polymorphic DNA (RAPD) analysis, the imipenem-resistant isolates displayed a relatively high degree of genetic variability and were unlikely associated with nosocomial infections.
对 32 株分离自住院儿童的耐亚胺培南铜绿假单胞菌临床分离株的碳青霉烯类耐药机制进行了研究。序列分析显示，31 株分离株的插入序列元件 ISRP10 破坏了孔蛋白基因 oprD，表明 ISRP10 对 oprD 的失活使大多数分离株对亚胺培南产生耐药性。多位点序列分型 (MLST) 用于区分分离株。共鉴定出 11 种序列型 (ST)，包括 3 种新的 ST，68.3%（28/41）的受试菌株被鉴定为克隆 ST253。与随机扩增多态性 DNA (RAPD) 分析相结合，耐亚胺培南的分离株显示出相对较高的遗传变异性，不太可能与医院感染有关。
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