Fiamingo Anderson, Montembault Alexandra, Boitard Solène-Emmanuelle, Naemetalla Hany, Agbulut Onnik, Delair Thierry, Campana-Filho Sérgio Paulo, Menasché Philippe, David Laurent
Ingénierie des Matériaux Polymères IMP@Lyon1, Univ Lyon, Université Claude Bernard Lyon 1, UMR CNRS 5223 , 15 bd Latarjet, 69622 Villeurbanne Cedex, France.
Instituto de Química de São Carlos, Universidade de São Paulo , Avenida Trabalhador São-carlense, 400 São Carlos, Brazil.
Biomacromolecules. 2016 May 9;17(5):1662-72. doi: 10.1021/acs.biomac.6b00075. Epub 2016 Apr 25.
The formation of chitosan hydrogels without any external cross-linking agent was successfully achieved by inducing the gelation of a viscous chitosan solution with aqueous NaOH or gaseous NH3. The hydrogels produced from high molecular weight (Mw ≈ 640 000 g mol(-1)) and extensively deacetylated chitosan (DA ≈ 2.8%) at polymer concentrations above ∼2.0% exhibited improved mechanical properties due to the increase of the chain entanglements and intermolecular junctions. The results also show that the physicochemical and mechanical properties of chitosan hydrogels can be controlled by varying their polymer concentration and by controlling the gelation conditions, that is, by using different gelation routes. The biological evaluation of such hydrogels for regeneration of infarcted myocardium revealed that chitosan hydrogels prepared from 1.5% polymer solutions were perfectly incorporated onto the epicardial surface of the heart and presented partial degradation accompanied by mononuclear cell infiltration.
通过用NaOH水溶液或气态NH3诱导粘性壳聚糖溶液凝胶化，成功实现了无需任何外部交联剂的壳聚糖水凝胶的形成。在聚合物浓度高于约2.0%时，由高分子量（Mw≈640 000 g mol(-1)）和高度脱乙酰化的壳聚糖（DA≈2.8%）制备的水凝胶由于链缠结和分子间连接的增加而表现出改善的机械性能。结果还表明，壳聚糖水凝胶的物理化学和机械性能可以通过改变其聚合物浓度以及控制凝胶化条件来控制，即通过使用不同的凝胶化途径。对这种用于梗死心肌再生的水凝胶的生物学评估表明，由1.5%聚合物溶液制备的壳聚糖水凝胶完美地整合到心脏的心外膜表面，并呈现出部分降解，伴有单核细胞浸润。
