Naik V K, Shakya S, Patyal A, Gade N E
Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Chhattisgarh Kamdhenu Vishwa Vidyalaya, Anjora, Durg, Chhattisgarh, India.
Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Chhattisgarh Kamdhenu Vishwa Vidyalaya, Anjora, Durg, Chhattisgarh, India.
Vet World. 2015 Jun;8(6):702-6. doi: 10.14202/vetworld.2015.702-706. Epub 2015 Jun 6.
The aim was to assess the prevalence of Salmonella in raw chevon and chicken meat sold in the retail meat shops situated in and around Durg, Rajnandgaon, Dhamtari, Raipur, and Bilaspur districts of Chhattisgarh. Studies were also conducted to find out the antibiotic resistance in Salmonella isolates.
A total of 400 samples comprising of 200 chevon meat and 200 chicken meat samples were processed for isolation of Salmonella and all isolates were further confirmed on the basis of cultural and biochemical characters and by targeting invA gene of Salmonella. All Salmonella isolates were also examined for their antimicrobial drug susceptibility/resistance pattern against commonly used antibiotics.
Out of 400 samples, the prevalence of Salmonella in chevon and chicken meat was found 9% and 7% respectively, with an overall prevalence of 8%. Polymerase chain reaction targeting invA gene of Salmonella showed positive result with 31 isolates. All 32 Salmonella isolates were found to be highly sensitive to ciprofloxacin while 96.87%, 96.87% and 93.75% were sensitive to gentamicin, imipenem, and ceftazidime, respectively. 93.75% and 59.37% isolates were resistant to erythromycin and oxytetracycline, respectively. Out of 32, 14 isolates had multiple antibiotic resistance index equal to or more than 0.2.
Salmonella in chevon and chicken meat samples is prevailing in the areas of sampling due to poor hygienic conditions and also demonstrated the varied spectrum of antimicrobial resistance, including several multiple drug resistance phenotypes. Therefore, the present study emphasizes the need for continued surveillance of zoonotic foodborne pathogens including antimicrobial-resistant variants throughout the food production chain.
评估在恰蒂斯加尔邦杜尔格、赖金德冈、达姆塔里、赖布尔和比拉斯布尔地区及周边零售肉店所售生山羊肉和鸡肉中沙门氏菌的流行情况。还开展了研究以查明沙门氏菌分离株的抗生素耐药性。
共处理400份样本，其中包括200份山羊肉样本和200份鸡肉样本，用于沙门氏菌分离，所有分离株均根据培养和生化特征以及通过靶向沙门氏菌的invA基因进行进一步确认。还对所有沙门氏菌分离株针对常用抗生素的抗菌药物敏感性/耐药模式进行了检测。
在400份样本中，山羊肉和鸡肉中沙门氏菌的流行率分别为9%和7%，总体流行率为8%。靶向沙门氏菌invA基因的聚合酶链反应对31株分离株显示阳性结果。所有32株沙门氏菌分离株对环丙沙星高度敏感，而对庆大霉素、亚胺培南和头孢他啶的敏感率分别为96.87%、96.87%和93.75%。分离株对红霉素和土霉素的耐药率分别为93.75%和59.37%。在32株分离株中，有14株的多重抗生素耐药指数等于或大于0.2。
由于卫生条件差，山羊肉和鸡肉样本中的沙门氏菌在采样地区普遍存在，并且还表现出不同的抗菌耐药谱，包括几种多重耐药表型。因此，本研究强调需要对整个食品生产链中的人畜共患食源性病原体（包括抗菌耐药变体）进行持续监测。
