Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.
Vet Med Sci. 2022 Jan;8(1):229-236. doi: 10.1002/vms3.647. Epub 2021 Oct 1.
Zoonotic food-borne pathogens such as Salmonella spp., which can be hosted by some raw foods, play a crucial role in ranking the public health of a country OBJECTIVES: The present study was conducted to assess the frequency, antibiotic resistance pattern and index of multiple antibiotic resistance (MAR) of Salmonella spp. in chicken meat METHODS: A cross-sectional survey was conducted from October 2017 to March 2018. One-hundred and fifty chicken meat samples were collected from meat stores in Zahedan, southeast of Iran and screened for contamination with Salmonella spp. using the polymerase chain reaction assay targeting the inv-A gene. Antimicrobial susceptibility testing was performed against 11 commonly prescribed antimicrobial agents in the veterinary treatment to calculate the MAR index RESULTS: The contamination rate was 2.7% (4/150). The antimicrobial resistance rate was 100% (n = 4) against penicillin, tylosin, tetracycline, erythromycin and tiamulin, 50% (n = 2) against trimethoprim/sulfamethoxazole, difloxacin and lincomycin/spectinomycin and 25% (n = 1) against flumequine and florfenicol. All isolates were sensitive to fosfomycin. Interestingly, all isolates (n = 4) exhibited different MAR patterns. Furthermore, the MAR index ranged from 0.45 to 0.81 CONCLUSIONS: In addition to the MAR index, which indicated that the isolate originated from a source where antibiotics were used to a great degree and/or in large amounts, the results showed that the chicken meat hosted resistant strains of Salmonella spp. in the study area. Overall, the findings indicated an important public health problem. To reduce this alarming signal, the poultry industry should implement control measures in the study area.
动物性食品源病原菌，如沙门氏菌，可寄生于某些生食，在国家公共卫生风险评估中占有重要地位。目的：本研究旨在评估伊朗东南部扎黑丹鸡肉中沙门氏菌的频率、抗生素耐药模式和多重抗生素耐药指数（MAR）。方法：采用横断面研究，于 2017 年 10 月至 2018 年 3 月期间，从扎黑丹市的肉店采集 150 份鸡肉样本，采用聚合酶链反应（PCR）检测 inv-A 基因检测沙门氏菌污染。对兽医治疗中常用的 11 种抗生素进行药敏试验，计算 MAR 指数。结果：污染率为 2.7%（4/150）。对青霉素、泰乐菌素、四环素、红霉素和替米考星的耐药率为 100%（n=4），对复方磺胺甲噁唑、二氟沙星和林可霉素/大观霉素的耐药率为 50%（n=2），对三甲氧苄氨嘧啶/磺胺甲噁唑、氟苯尼考和氟喹诺酮的耐药率为 25%（n=1）。所有分离株对磷霉素均敏感。有趣的是，所有分离株（n=4）均表现出不同的 MAR 模式。此外，MAR 指数范围为 0.45 至 0.81。结论：除了 MAR 指数表明分离株来自抗生素大量和/或高频率使用的来源外，结果表明研究地区的鸡肉中存在耐药性沙门氏菌。总体而言，这些发现表明存在一个重要的公共卫生问题。为了降低这一警报信号，研究地区的家禽业应采取控制措施。
