用于检测甲型流感病毒的基于巨磁阻的生物传感器。
Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus.
作者信息
Krishna Venkatramana D, Wu Kai, Perez Andres M, Wang Jian-Ping
机构信息
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul MN, USA.
Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis MN, USA.
出版信息
Front Microbiol. 2016 Mar 29;7:400. doi: 10.3389/fmicb.2016.00400. eCollection 2016.
We have developed a simple and sensitive method for the detection of influenza A virus based on giant magnetoresistance (GMR) biosensor. This assay employs monoclonal antibodies to viral nucleoprotein (NP) in combination with magnetic nanoparticles (MNPs). Presence of influenza virus allows the binding of MNPs to the GMR sensor and the binding is proportional to the concentration of virus. Binding of MNPs onto the GMR sensor causes change in the resistance of sensor, which is measured in a real time electrical readout. GMR biosensor detected as low as 1.5 × 10(2) TCID50/mL virus and the signal intensity increased with increasing concentration of virus up to 1.0 × 10(5) TCID50/mL. This study showed that the GMR biosensor assay is relevant for diagnostic application since the virus concentration in nasal samples of influenza virus infected swine was reported to be in the range of 10(3) to 10(5) TCID50/mL.
我们基于巨磁电阻（GMR）生物传感器开发了一种简单且灵敏的甲型流感病毒检测方法。该检测方法采用针对病毒核蛋白（NP）的单克隆抗体，并结合磁性纳米颗粒（MNP）。流感病毒的存在使得MNP与GMR传感器结合，且这种结合与病毒浓度成正比。MNP与GMR传感器的结合会导致传感器电阻发生变化，可通过实时电读出进行测量。GMR生物传感器能够检测低至1.5×10² TCID50/mL的病毒，并且信号强度随着病毒浓度增加至1.0×10⁵ TCID50/mL而增强。该研究表明，GMR生物传感器检测方法适用于诊断应用，因为据报道，感染流感病毒的猪鼻腔样本中的病毒浓度在10³至10⁵ TCID50/mL范围内。
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