Department of Recombinant Vaccine, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822 Gdansk, Poland.
Department of Recombinant Vaccine, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822 Gdansk, Poland.
Biosens Bioelectron. 2014 Sep 15;59:239-42. doi: 10.1016/j.bios.2014.03.050. Epub 2014 Apr 1.
Influenza is a contagious disease caught by humans caused by viruses belonging to the family Orthomyxoviridae. Each year, the influenza virus infects millions of people and kills hundreds of thousands of them. Traditional diagnostic methods, such as virus propagation and isolation, antigen capture immunoassays and molecular methods are not sufficient for the detection of the influenza virus. Development of a valid diagnostic assay for quick detection (in less than an hour) of the virus, with high sensitivity, is a challenge for researchers all over the world. Here we present a new, universal immunosensor for detection of the influenza A virus. By using electrochemical impedance spectroscopy (EIS) and direct attachment of antibodies to the gold electrode the assay allows detection of the pathogen with sensitivity similar to molecular methods in relatively short time. Application of universal anti-M1 antibodies allows detection of all serotypes of influenza A virus. The simple design of the sensor facilitates miniaturization of the device and its implementation for routine diagnostics during first contact with the patient, before applying a proper treatment.
流感是一种由正粘病毒科病毒引起的人类传染病。每年，流感病毒都会感染数百万人，并导致数十万人死亡。传统的诊断方法，如病毒繁殖和分离、抗原捕获免疫测定和分子方法，不足以检测流感病毒。开发一种有效的诊断方法，用于快速（不到一小时）检测病毒，具有高灵敏度，是全世界研究人员的一个挑战。在这里，我们提出了一种新的、通用的流感 A 病毒免疫传感器。通过使用电化学阻抗谱（EIS）和直接将抗体附着到金电极上，该检测方法允许以类似于分子方法的灵敏度在相对较短的时间内检测病原体。通用抗 M1 抗体的应用允许检测所有类型的流感 A 病毒。传感器的简单设计便于设备的小型化，并可在与患者首次接触时进行常规诊断，然后再进行适当的治疗。
