Mlynáriková Katarína, Šedo Ondrej, Růžička Filip, Zdráhal Zbyněk, Holá Veronika, Mahelová Martina
Department of Microbiology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, 62500, Czech Republic.
Research Group Proteomics, Central European Institute of Technology, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic.
Folia Microbiol (Praha). 2016 Nov;61(6):465-471. doi: 10.1007/s12223-016-0458-7. Epub 2016 Apr 11.
Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is, currently, used as a rapid and reliable tool in microbial diagnostics. The discriminatory power of the method extends its applicability also beyond species level. This study examined the possibility to use MALDI-TOF MS to differentiate between Candida parapsilosis sensu stricto biofilm-positive (n = 12) and biofilm-negative (n = 9) strains. The results indicated a grouping trend within MALDI-TOF mass spectra belonging to each of the tested groups. However, these trends were eclipsed by mass spectral variations resulting from limited repeatability of the method, making its application for the selected purpose impossible. Improvement in the discriminatory power of the method was not obtained neither by using different matrices (α-cyano-4-hydroxycinnamic acid, ferulic acid, 5-chloro-2-mercaptobenzothionazole) for MALDI-TOF MS analysis nor by testing different culture conditions (cultivation length, culture media).
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）目前在微生物诊断中用作一种快速且可靠的工具。该方法的鉴别能力使其适用性也扩展到物种水平之外。本研究考察了使用MALDI-TOF MS区分近平滑念珠菌狭义生物膜阳性菌株（n = 12）和生物膜阴性菌株（n = 9）的可能性。结果表明，在属于每个测试组的MALDI-TOF质谱图中存在分组趋势。然而，由于该方法重复性有限导致的质谱变化掩盖了这些趋势，使得其无法用于选定的目的。无论是通过使用不同的基质（α-氰基-4-羟基肉桂酸、阿魏酸、5-氯-2-巯基苯并噻唑）进行MALDI-TOF MS分析，还是通过测试不同的培养条件（培养时长、培养基），都未实现该方法鉴别能力的提升。
