来自HeLa细胞紫外线转录图谱的证据表明，不均一核RNA是信使RNA前体。
Evidence from UV transcription mapping in HeLa cells that heterogeneous nuclear RNA is the messenger RNA precursor.
作者信息
Goldberg S, Schwartz H, Darnell J E
出版信息
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4520-3. doi: 10.1073/pnas.74.10.4520.
Abstract
The effects of UV irradiation on the incorporation of [3H]uridine in HeLa (human) cell mRNA, rRNA, heterogeneous nuclear RNA (hnRNA) and early mRNA from adenovirus type 2 have been compared. The UV target size of cell mRNA is at least 3 times larger than the average size of the mRNA itself and larger than the adenovirus-2 early mRNA, which is known to derive from transcription units of about 1.5-5.0 kilobases. The UV target size of hnRNA, in contrast, is about the same as its size determined by sedimentation and overlaps with the target size of mRNA. It is concluded that most mRNA derives from a higher molecular weight hnRNA molecule.
摘要
已比较了紫外线照射对[3H]尿苷掺入HeLa（人）细胞信使核糖核酸（mRNA）、核糖体核糖核酸（rRNA）、不均一核核糖核酸（hnRNA）以及腺病毒2型早期mRNA的影响。细胞mRNA的紫外线靶标大小至少比mRNA自身的平均大小大3倍，且大于腺病毒2型早期mRNA，已知腺病毒2型早期mRNA源自约1.5 - 5.0千碱基的转录单位。相比之下，hnRNA的紫外线靶标大小与其通过沉降法测定的大小大致相同，且与mRNA的靶标大小重叠。得出的结论是，大多数mRNA源自分子量更高的hnRNA分子。
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