Yssouf Amina, Almeras Lionel, Raoult Didier, Parola Philippe
Aix Marseille Université, Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, Faculté de Médecine, 27 bd Jean Moulin, 13385 Marseille cedex 5, France.
Future Microbiol. 2016;11(4):549-66. doi: 10.2217/fmb.16.5. Epub 2016 Apr 12.
The rapid and reliable identification of arthropod vector species is an essential component of the fight against vector-borne diseases. However, owing to the lack of entomological expertise required for the morphological identification method, development of alternative and complementary tools is needed. This review describes the main methods used for arthropod identification, focusing on the emergence of protein profiling using MALDI-TOF MS technology. Sample preparation, analysis of reproducibility, database creation and blind tests for controlling accuracy of this tool for arthropod identification are described. The advantages and limitations of the MALDI-TOF MS method are illustrated by emphasizing different hematophagous arthropods, including mosquitoes and ticks, the top two main vectors of infectious diseases.
快速且可靠地鉴定节肢动物病媒物种是抗击病媒传播疾病的重要组成部分。然而,由于形态学鉴定方法需要昆虫学专业知识,因此需要开发替代和补充工具。本综述描述了用于节肢动物鉴定的主要方法,重点关注使用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术进行蛋白质谱分析的出现情况。描述了样本制备、重现性分析、数据库创建以及用于控制该节肢动物鉴定工具准确性的盲法测试。通过强调不同的吸血节肢动物,包括蚊子和蜱虫这两种主要的传染病病媒,说明了MALDI-TOF MS方法的优点和局限性。