Evans F E, Kaplan N O
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4909-13. doi: 10.1073/pnas.74.11.4909.
A survey of phosphorus compounds present in HeLa cells and their acid extracts has been carried out by (31)P nuclear magnetic resonance spectroscopy at 40 MHz. The proton decoupled (31)P spectrum of the neutralized extract had resolution adequate to enable the identification of the main phosphate compounds. The spectral intensities were converted to concentrations. The lower detection limit with extensive signal averaging was 0.02 mumol for the extract. The composition, listed in order of decreasing concentration, was: inorganic phosphate, ATP, phosphorylcholine, creatine phosphate, UTP, NAD(+), glucose 6-phosphate, beta-D-fructose 1,6-bisphosphate, alpha-D-fructose 1,6-bisphosphate, ADP, alpha-glycerophosphorylcholine, and alpha-glycerophosphorylethanolamine. UTP made up (1/5) of the total nucleotide triphosphate content. The composition was compared to the (31)P spectrum of an extract from a human astrocytoma grown in athymic mice. The signal from P-containing macromolecules such as nucleic acids was not detected in the intact HeLa cell spectrum because of broad lines. Effects of the glycolysis inhibitor iodoacetic acid could be clearly shown in spectra of both the intact cell and the extract as buildup of fructose 1,6-bisphosphate at the expense of ATP, UTP, and creatine phosphate.
利用40兆赫的磷-31核磁共振波谱法对海拉细胞及其酸提取物中存在的磷化合物进行了一项调查。中和提取物的质子去耦磷-31谱具有足够的分辨率，能够鉴定主要的磷酸盐化合物。光谱强度被转换为浓度。经过大量信号平均后的最低检测限为提取物0.02微摩尔。按浓度递减顺序列出的成分是：无机磷酸盐、三磷酸腺苷（ATP）、磷酸胆碱、磷酸肌酸、三磷酸尿苷（UTP）、烟酰胺腺嘌呤二核苷酸（NAD⁺）、6-磷酸葡萄糖、β-D-果糖1,6-二磷酸、α-D-果糖1,6-二磷酸、二磷酸腺苷（ADP）、α-甘油磷酸胆碱和α-甘油磷酸乙醇胺。UTP占总核苷酸三磷酸含量的五分之一。将该成分与在无胸腺小鼠中生长的人类星形细胞瘤提取物的磷-31谱进行了比较。由于谱线较宽，在完整的海拉细胞谱中未检测到来自含磷大分子（如核酸）的信号。糖酵解抑制剂碘乙酸的作用在完整细胞和提取物的谱中都能清楚地显示出来，即果糖1,6-二磷酸的积累以消耗ATP、UTP和磷酸肌酸为代价。
