通过高分辨率31P核磁共振观察淋巴细胞、Friend红白血病细胞和HeLa细胞中的磷酸盐代谢物。
Phosphate metabolites in lymphoid, Friend erythroleukemia, and HeLa cells observed by high-resolution 31P nuclear magnetic resonance.
作者信息
Navon G, Navon R, Shulman R G, Yamane T
出版信息
Proc Natl Acad Sci U S A. 1978 Feb;75(2):891-5. doi: 10.1073/pnas.75.2.891.
Abstract
High-resolution phosphorus-31 nuclear magnetic resonance spectra were observed for cell lines from mammalian sources: lymphoid cells, Friend erythroleukemia cells, and HeLa cells. Some phosphate metabolites were identified and their concentrations were found to vary among the different cell lines. ATP/ADP ratios could be measured in the living cells. The phospholipid metabolites phosphorylcholine and phosphorylethanolamine and their glyceryl esters were present in relatively high concentrations in the cells, but their ratios varied considerably. Also, a high concentration of an unidentified diphosphodiester compound was observed in the Friend erythroleukemia and HeLa cells. No appreciable pH gradient across the cell membrance was observed in these three mammalian cell lines, in contrast to the large pH gradient detected in microorganisms.
摘要
观察到了来自哺乳动物的细胞系的高分辨率磷-31核磁共振光谱：淋巴细胞、弗氏红白血病细胞和HeLa细胞。鉴定出了一些磷酸盐代谢物，发现它们在不同细胞系中的浓度有所不同。可以在活细胞中测量ATP/ADP比率。磷脂代谢物磷酸胆碱和磷酸乙醇胺及其甘油酯在细胞中以相对较高的浓度存在，但它们的比率差异很大。此外，在弗氏红白血病细胞和HeLa细胞中观察到高浓度的一种未鉴定的二磷酸二酯化合物。与在微生物中检测到的大pH梯度相反，在这三种哺乳动物细胞系中未观察到跨细胞膜的明显pH梯度。
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