完整人体红细胞中的磷酸盐代谢：通过磷-31核磁共振光谱法测定
Phosphate metabolism in intact human erythrocytes: determination by phosphorus-31 nuclear magnetic resonance spectroscopy.
作者信息
Henderson T O, Costello A J, Omachi A
出版信息
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2487-90. doi: 10.1073/pnas.71.6.2487.
Abstract
Whole human blood was examined by (31)P nuclear magnetic resonance spectroscopy. Individual phosphates (alpha,beta,gamma) of ATP were identifiable, and two microenvironments appeared to be present for this molecule. When sequential recordings of freshly collected blood were made, 2,3-diphosphoglycerate was observed to decrease in association with a concomitant increase in inorganic orthophosphate. When aged cells containing little 2,3-diphosphoglycerate were incubated in the presence of inosine and pyruvate, 2,3-diphosphoglycerate formation could be demonstrated. These results show that cellular metabolism can be recorded directly in intact cells by (31)P nuclear magnetic resonance.
摘要
采用³¹P核磁共振波谱法对全血进行检测。ATP的各个磷酸基团（α、β、γ）均可识别，该分子似乎存在两种微环境。对新鲜采集的血液进行连续记录时，观察到2,3-二磷酸甘油酸减少，同时无机正磷酸盐增加。当将含少量2,3-二磷酸甘油酸的衰老细胞在肌苷和丙酮酸存在的情况下进行孵育时，可证明有2,3-二磷酸甘油酸生成。这些结果表明，通过³¹P核磁共振可直接在完整细胞中记录细胞代谢情况。
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