Shu Yachun, Chen Yajun, Qin Kunming, Liu Xiao, Cai Baochang
Department of Pharmacy, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Department of Pharmacy, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.
Pharmacogn Mag. 2016 Apr-Jun;12(46):134-8. doi: 10.4103/0973-1296.177911.
Yinqiaosan (Lonicerae and Forsythiae Powder), as a famous prescription of Dr. Wu Jutong in Qing dynasty of China, has the effects of diaphoresis cooling, fire-purging, and detoxicaton. It is mainly used in the treatment of influenza, hand-foot-mouth disease, esophagitis, pneumonia, acute tonsillitis, mumps, and other viral infections. It is one of the widely used traditional Chinese medicine prescriptions with proven curative effects in clinical use.
To research the material basis of Yinqiaosan decoction when decocting mint, herba schizonepetae in different length of later-decoction time, to find the influence on volatile components of Yinqiaosan decoction decocted later in different length of time, to lay the foundation to further clarify the after-decoction mechanism of Yinqiaosan, and the specification of Yinqiaosan decoction process.
Gas chromatography mass spectrometry method is used to analyze the volatile components of Yinqiaosan decoction samples decocted for 0, 3, 5, 8, and 10 min.
Later-decocting mint and herba schizonepetae at different time when decocting Yinqiaosan had a significant influence on the volatile components of the solution. 54 different chemical components were identified: 25 were identified when later-decocting the sample for 3 min; 13 were identified when later-decocting the sample for 5 min; 11 were identified when later-decocting the sample for 8 min; 7 were identified when later-decocting the sample for 10 min; and 26 were identified when later-decocting the sample for 0 min. There were more volatile components in the sample after-decocted for 3 min. A total of 54 different chemical components were identified in different later-decocting solution samples. These components form the basis of the Yinqiaosan drug effect.
The length of later-decoction time of mint and herba schizonepetae was confirmed to be 3 min when decocting Yinqiaosan.
Later-decocting mint and herba schizonepetae at different time had a significant influence on the volatile components of the solutionFifty-four different chemical components were identified in different later-decocting solution samplesThere were more volatile components in the sample after-decocted for 3 minThe volatile components content was high. These components form the important basis of the Yinqiaosan drug effect.Total ion flow diagram of volatile oils in the Yinqiaosan sample with mint, herba schizonepetae after 3 min decoction. Abbreviations used: GC-MS: Gas chromatography mass spectrometry, TCM: Traditional Chinese medicine.
银翘散是中国清代吴鞠通的名方，具有辛凉透表、清热解毒之功效。主要用于治疗流感、手足口病、食管炎、肺炎、急性扁桃体炎、腮腺炎等病毒感染性疾病。是临床应用中疗效确切、应用广泛的中药方剂之一。
研究银翘散煎煮时薄荷、荆芥后下不同时长的物质基础，探讨不同时长后下对银翘散汤剂挥发油成分的影响，为进一步阐明银翘散后下机制及银翘散汤剂工艺规范奠定基础。
采用气相色谱-质谱联用方法分析银翘散汤剂分别煎煮0、3、5、8、10分钟的样品挥发油成分。
银翘散煎煮时不同时间后下薄荷、荆芥对溶液挥发油成分有显著影响。共鉴定出54种不同化学成分：后下3分钟的样品鉴定出25种；后下5分钟的样品鉴定出13种；后下8分钟的样品鉴定出11种；后下10分钟的样品鉴定出7种；后下0分钟的样品鉴定出26种。后下3分钟的样品挥发油成分较多。不同后下溶液样品共鉴定出54种不同化学成分。这些成分构成了银翘散药效的基础。
银翘散煎煮时薄荷、荆芥后下时长确定为3分钟。
银翘散煎煮时不同时间后下薄荷、荆芥对溶液挥发油成分有显著影响。不同后下溶液样品共鉴定出54种不同化学成分。后下3分钟的样品挥发油成分较多，挥发油成分含量高。这些成分构成了银翘散药效的重要基础。银翘散含薄荷、荆芥煎煮3分钟后挥发油的总离子流图。所用缩写：GC-MS：气相色谱-质谱联用，TCM：中药
