Ito Y, Oka H, Lee Y W
Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
J Chromatogr. 1990 Jan 5;498(1):169-78. doi: 10.1016/s0021-9673(01)84245-x.
The semipreparative capability of the newly developed high-speed counter-current chromatograph equipped with a set of three multilayer coils has been demonstrated in separations of a variety of biological samples including triterpenoic acids, indole auxins, bacitracin, flavonoids and tetracycline derivatives, each with a suitable two-phase solvent system. The sample quantities ranging from 50 to 500 mg were efficiently separated within a few hours. The separation of tetracycline derivatives was remarkably improved by adding ammonium acetate to the solvent system.
配备一组三个多层线圈的新开发的高速逆流色谱仪的半制备能力已在多种生物样品的分离中得到证明，这些样品包括三萜酸、吲哚生长素、杆菌肽、黄酮类化合物和四环素衍生物，每种样品都使用合适的两相溶剂系统。50至500毫克的样品量在数小时内得到有效分离。通过向溶剂系统中加入醋酸铵，四环素衍生物的分离得到了显著改善。
