Kollia Eleni, Tsourouflis Kyriakos, Markaki Panagiota
a Department of Chemistry, Laboratory of Food Chemistry , National and Kapodistrian University of Athens , Athens , Greece.
Food Addit Contam Part B Surveill. 2016 Sep;9(3):217-22. doi: 10.1080/19393210.2016.1179349. Epub 2016 May 17.
Aflatoxin B1 (AFB1) is considered as the most potent liver carcinogen for humans. A method for determination in sesame seeds was developed. AFB1 was extracted by methanol-water, cleaned by immunoaffinity columns and determined by high-performance liquid chromatography with fluorescence detection. The recovery factor and the limit of detection (LOD) of AFB1 in sesame seeds were 111.5% and 0.02 ng g(-1), respectively. Thirty samples of sesame products were examined for the presence of AFB1. After analysis, 77.6% of samples were found to be contaminated. Eight samples exceeded the European Union (EU) limit (2 µg AFB1 kg(-1)). In 15 samples, AFB1 was below the EU limit. Seven samples remained below the LOD. The most contaminated (14.49 ng AFB1 g(-1)) sample was unpeeled packaged sesame seeds. In all samples, aflatoxigenic Aspergilli fungi as well as the risk for AFB1 presence in sesame seed was investigated.
黄曲霉毒素B1（AFB1）被认为是对人类最具致癌性的肝脏致癌物。开发了一种测定芝麻中AFB1的方法。AFB1用甲醇 - 水提取，通过免疫亲和柱净化，并用带荧光检测的高效液相色谱法测定。芝麻中AFB1的回收率和检测限（LOD）分别为111.5%和0.02 ng g(-1)。对30个芝麻产品样品进行了AFB1检测。分析后发现，77.6%的样品受到污染。8个样品超过了欧盟（EU）限量（2 µg AFB1 kg(-1)）。15个样品中的AFB1低于欧盟限量。7个样品低于检测限。污染最严重的（14.49 ng AFB1 g(-1)）样品是带壳包装芝麻籽。在所有样品中，对产黄曲霉的曲霉菌以及芝麻籽中存在AFB1的风险进行了调查。
