Ray Sandipan, Patel Sandip K, Venkatesh Apoorva, Bhave Amruta, Kumar Vipin, Singh Vaidhvi, Chatterjee Gangadhar, Shah Veenita G, Sharma Sarthak, Renu Durairaj, Nafis Naziya, Gandhe Prajakta, Gogtay Nithya, Thatte Urmila, Sehgal Kunal, Verma Sumit, Karak Avik, Khanra Dibbendhu, Talukdar Arunansu, Kochar Sanjay K, S B Vijeth, Kochar Dhanpat K, Rojh Dharmendra, Varma Santosh G, Gandhi Mayuri N, Srikanth Rapole, Patankar Swati, Srivastava Sanjeeva
Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Dept of Biochemistry, Grant Govt Medical College and Sir JJ Group of Hospitals, Byculla, Mumbai 400008, India.
Sci Rep. 2016 Apr 19;6:24557. doi: 10.1038/srep24557.
In Plasmodium vivax malaria, mechanisms that trigger transition from uncomplicated to fatal severe infections are obscure. In this multi-disciplinary study we have performed a comprehensive analysis of clinicopathological parameters and serum proteome profiles of vivax malaria patients with different severity levels of infection to investigate pathogenesis of severe malaria and identify surrogate markers of severity. Clinicopathological analysis and proteomics profiling has provided evidences for the modulation of diverse physiological pathways including oxidative stress, cytoskeletal regulation, lipid metabolism and complement cascades in severe malaria. Strikingly, unlike severe falciparum malaria the blood coagulation cascade was not found to be affected adversely in acute P. vivax infection. To the best of our knowledge, this is the first comprehensive proteomics study, which identified some possible cues for severe P. vivax infection. Our results suggest that Superoxide dismutase, Vitronectin, Titin, Apolipoprotein E, Serum amyloid A, and Haptoglobin are potential predictive markers for malaria severity.
在间日疟原虫疟疾中，引发从非复杂性感染转变为致命性严重感染的机制尚不清楚。在这项多学科研究中，我们对不同感染严重程度的间日疟患者的临床病理参数和血清蛋白质组图谱进行了全面分析，以研究严重疟疾的发病机制并确定严重程度的替代标志物。临床病理分析和蛋白质组学分析为严重疟疾中包括氧化应激、细胞骨架调节、脂质代谢和补体级联反应在内的多种生理途径的调节提供了证据。令人惊讶的是，与严重恶性疟不同，在急性间日疟感染中未发现凝血级联反应受到不利影响。据我们所知，这是第一项全面的蛋白质组学研究，它确定了一些严重间日疟感染的可能线索。我们的结果表明，超氧化物歧化酶、玻连蛋白、肌联蛋白、载脂蛋白E、血清淀粉样蛋白A和触珠蛋白是疟疾严重程度的潜在预测标志物。
