Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, U.P., India.
Department of Microbiology, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh, U.P., India.
Parasitol Res. 2021 Apr;120(4):1437-1446. doi: 10.1007/s00436-021-07065-3. Epub 2021 Feb 3.
Severe complications have been observed and established for Plasmodium falciparum as well as P. vivax infections worldwide. Although P. vivax infection is not fully acknowledged as malignant malaria, recently life-threatening complications have been reported to occur in many studies. The recognition of biomarkers with excellent sensitivity and reliability plays a prime role in disease management. Acute inflammatory response and oxidative stress are observed in malaria due to the production of reactive oxygen species. Lipid and protein oxidative injuries are prospective biomarkers for disease severity owing to the damage caused by the parasite. We have tried to find out whether protein carbonylation (PC), lipid peroxidation (LPO) and superoxide dismutase (SOD) could suffice as a biomarker for severe vivax malaria or not. Blood samples were collected from the individuals attending Jawaharlal Nehru Medical College of Aligarh Muslim University during the wet season of malaria transmission. Microscopy and rapid diagnostic kits were used as a tool for malaria diagnosis. A total of 214 subjects were enrolled for the study: 30 febrile controls and 184 subjects with vivax malaria. Protein carbonylation and lipid peroxidation were found to be directly associated with parasite count and total antioxidant status (TAS). Increase in oxidative stress was also observed in severe vivax malaria patients. Levels of uric acid and bilirubin too were raised in complicated cases. Protein carbonylation was found to be a more reliable indicator of vivax malaria severity than lipid peroxidation.
严重并发症已在全世界范围内观察到并确立了恶性疟原虫和间日疟原虫感染。虽然间日疟感染未被完全认定为恶性疟疾，但最近许多研究报告了危及生命的并发症。具有出色敏感性和可靠性的生物标志物的识别在疾病管理中起着主要作用。由于活性氧的产生，疟疾中会观察到急性炎症反应和氧化应激。由于寄生虫引起的损伤，脂质和蛋白质氧化损伤是疾病严重程度的潜在生物标志物。我们试图找出蛋白羰基化（PC）、脂质过氧化（LPO）和超氧化物歧化酶（SOD）是否可以作为严重间日疟的生物标志物。在疟疾传播的雨季，从阿拉哈巴德穆斯林大学贾瓦哈拉尔尼赫鲁医学院就诊的个体中采集血液样本。显微镜和快速诊断试剂盒被用作疟疾诊断的工具。共有 214 名受试者参加了这项研究：30 名发热对照组和 184 名间日疟组。蛋白羰基化和脂质过氧化与寄生虫计数和总抗氧化状态（TAS）直接相关。严重间日疟患者的氧化应激也增加。胆红素和尿酸水平在复杂病例中也升高。与脂质过氧化相比，蛋白羰基化被发现是间日疟严重程度更可靠的指标。
