Mello Priscila Luiza, Moraes Riboli Danilo Flávio, Pinheiro Luiza, de Almeida Martins Lisiane, Vasconcelos Paiva Brito Maria Aparecida, Ribeiro de Souza da Cunha Maria de Lourdes
Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu 18618-970, Brazil.
Department of Anatomic Pathology, Instituto Lauro de Souza Lima, Bauru 17034-971, Brazil.
Toxins (Basel). 2016 Apr 15;8(4):104. doi: 10.3390/toxins8040104.
Epidemiological studies have identified Staphylococcus aureus as the most common agent involved in food poisoning. However, current research highlights the importance of toxigenic coagulase-negative staphylococci (CoNS) isolated from food. The aim of this study was to characterize Staphylococcus spp. isolated from cows with bovine subclinical mastitis regarding the presence of genes responsible for the production of staphylococcal enterotoxins and of the tst-1 gene encoding toxic shock syndrome toxin 1, and to determine the clonal profile of the isolates carrying any of the genes studied. A total of 181 strains isolated in different Brazilian states, including the South, Southeast, and Northeast regions, were analyzed. The sea gene was the most frequent, which was detected in 18.2% of the isolates, followed by seb in 7.7%, sec in 14.9%, sed in 0.5%, see in 8.2%, seg in 1.6%, seh in 25.4%, sei in 6.6%, and ser in 1.6%. The sej, ses, set, and tst-1 genes were not detected in any of the isolates. The typing of the isolates by pulsed-field gel electrophoresis revealed important S. aureus and S. epidermidis clusters in different areas and the presence of enterotoxin genes in lineages isolated from animals that belong to herds located geographically close to each other.
流行病学研究已确定金黄色葡萄球菌是食物中毒中最常见的病原体。然而，目前的研究突出了从食品中分离出的产毒凝固酶阴性葡萄球菌（CoNS）的重要性。本研究的目的是对从患有牛亚临床乳腺炎的奶牛中分离出的葡萄球菌属菌株进行特征分析，以确定负责产生葡萄球菌肠毒素的基因以及编码中毒性休克综合征毒素1的tst-1基因的存在情况，并确定携带所研究任何基因的分离株的克隆谱。对在巴西不同州（包括南部、东南部和东北部地区）分离出的总共181株菌株进行了分析。sea基因最为常见，在18.2%的分离株中被检测到，其次是seb（7.7%）、sec（14.9%）、sed（0.5%）、see（8.2%）、seg（1.6%）、seh（25.4%）、sei（6.6%）和ser（1.6%）。在任何分离株中均未检测到sej、ses、set和tst-1基因。通过脉冲场凝胶电泳对分离株进行分型，揭示了不同地区重要的金黄色葡萄球菌和表皮葡萄球菌簇，以及在地理上彼此靠近的畜群中分离出的动物谱系中存在肠毒素基因。
