Hyman Jay M, Walsh John D, Ronsick Christopher, Wilson Mark, Hazen Kevin C, Borzhemskaya Larisa, Link John, Clay Bradford, Ullery Michael, Sanchez-Illan Mirta, Rothenberg Steven, Robinson Ron, van Belkum Alex, Dunne W Michael
bioMérieux Inc., Durham, North Carolina, USA.
Department of Pathology, Clinical Microbiology Laboratory, Duke University, Durham, North Carolina, USA.
mBio. 2016 Apr 19;7(2):e00491-16. doi: 10.1128/mBio.00491-16.
A clinical laboratory evaluation of an intrinsic fluorescence spectroscopy (IFS)-based identification system paired to a BacT/Alert Virtuo microbial detection system (bioMérieux, Inc., Durham, NC) was performed to assess the potential for fully automated identification of positive blood cultures. The prototype IFS system incorporates a novel method combining a simple microbial purification procedure with rapid in situ identification via spectroscopy. Results were available within 15 min of a bottle signaling positive and required no manual intervention. Among cultures positive for organisms contained within the database and producing acceptable spectra, 75 of 88 (85.2%) and 79 of 88 (89.8%) were correctly identified to the species and genus level, respectively. These results are similar to the performance of existing rapid methods.
A fully automated research platform was developed to identify microbial growth from positive blood cultures in <15 min. Because of the automated format, results can be generated during all shifts, with or without staffing, which in turn could promote more timely administration of target antimicrobial therapy.
对一种基于固有荧光光谱法（IFS）的鉴定系统与BacT/Alert Virtuo微生物检测系统（生物梅里埃公司，北卡罗来纳州达勒姆）配对进行了临床实验室评估，以评估全自动鉴定阳性血培养物的潜力。原型IFS系统采用了一种新颖的方法，将简单的微生物纯化程序与通过光谱法进行的快速原位鉴定相结合。在培养瓶发出阳性信号后15分钟内即可获得结果，且无需人工干预。在数据库中包含的、产生可接受光谱的生物体阳性培养物中，分别有88份中的75份（85.2%）和88份中的79份（89.8%）被正确鉴定到种和属水平。这些结果与现有快速方法的性能相似。
开发了一个全自动研究平台，可在不到15分钟的时间内从阳性血培养物中鉴定微生物生长。由于采用了自动化形式，无论是否有工作人员，在所有班次都能生成结果，这反过来又可以促进更及时地给予目标抗菌治疗。
