Rau J V, Fosca M, Graziani V, Egorov A A, Zobkov Yu V, Fedotov A Yu, Ortenzi M, Caminiti R, Baranchikov A E, Komlev V S
Istituto di Struttura della Materia, Consiglio Nazionale delle Ricerche, CNR-ISM, Via del Fosso del Cavaliere 100, Rome 00133, Italy.
A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Leninsky Prospect 49, Moscow 119334, Russia.
J Funct Biomater. 2016 Apr 18;7(2):10. doi: 10.3390/jfb7020010.
Calcium phosphate bone cements (CPCs) with antibacterial properties are demanded for clinical applications. In this study, we demonstrated the use of a relatively simple processing route based on preparation of silver-doped CPCs (CPCs-Ag) through the preparation of solid dispersed active powder phase. Real-time monitoring of structural transformations and kinetics of several CPCs-Ag formulations (Ag = 0 wt %, 0.6 wt % and 1.0 wt %) was performed by the Energy Dispersive X-ray Diffraction technique. The partial conversion of β-tricalcium phosphate (TCP) phase into the dicalcium phosphate dihydrate (DCPD) took place in all the investigated cement systems. In the pristine cement powders, Ag in its metallic form was found, whereas for CPC-Ag 0.6 wt % and CPC-Ag 1.0 wt % cements, CaAg(PO₃)₃ was detected and Ag (met.) was no longer present. The CPC-Ag 0 wt % cement exhibited a compressive strength of 6.5 ± 1.0 MPa, whereas for the doped cements (CPC-Ag 0.6 wt % and CPC-Ag 1.0 wt %) the reduced values of the compressive strength 4.0 ± 1.0 and 1.5 ± 1.0 MPa, respectively, were detected. Silver-ion release from CPC-Ag 0.6 wt % and CPC-Ag 1.0 wt % cements, measured by the Atomic Emission Spectroscopy, corresponds to the average values of 25 µg/L and 43 µg/L, respectively, rising a plateau after 15 days. The results of the antibacterial test proved the inhibitory effect towards pathogenic Escherichia coli for both CPC-Ag 0.6 wt % and CPC-Ag 1.0 wt % cements, better performances being observed for the cement with a higher Ag-content.
临床上需要具有抗菌性能的磷酸钙骨水泥（CPC）。在本研究中，我们展示了一种相对简单的加工路线，即通过制备固体分散活性粉末相来制备银掺杂的CPC（CPC-Ag）。采用能量色散X射线衍射技术对几种CPC-Ag配方（Ag = 0 wt%、0.6 wt%和1.0 wt%）的结构转变和动力学进行实时监测。在所有研究的水泥体系中，β-磷酸三钙（TCP）相部分转化为二水磷酸二钙（DCPD）。在原始水泥粉末中，发现了金属形式的Ag，而对于CPC-Ag 0.6 wt%和CPC-Ag 1.0 wt%水泥，检测到CaAg(PO₃)₃，且不再存在Ag（金属态）。CPC-Ag 0 wt%水泥的抗压强度为6.5±1.0 MPa，而对于掺杂水泥（CPC-Ag 0.6 wt%和CPC-Ag 1.0 wt%），分别检测到抗压强度降低值为4.0±1.0 MPa和1.5±1.0 MPa。通过原子发射光谱法测量，CPC-Ag 0.6 wt%和CPC-Ag 1.0 wt%水泥的银离子释放量分别对应于平均值25 µg/L和43 µg/L，15天后达到平稳状态。抗菌测试结果证明，CPC-Ag 0.6 wt%和CPC-Ag 1.0 wt%水泥对致病性大肠杆菌均有抑制作用，Ag含量较高的水泥表现出更好的性能。
