Kheirouri Sorayya, Hadi Vahid, Alizadeh Mohammad
a Department of Nutrition , Tabriz University of Medical Sciences , Tabriz , Iran.
Immunol Invest. 2016 May;45(4):271-83. doi: 10.3109/08820139.2016.1153649. Epub 2016 Apr 21.
Abundant evidence indicates the involvement of CD4(+), CD8(+), and CD4(+)CD25(+) T lymphocytes in the induction and/or protection of rheumatoid arthritis (RA). We aimed to investigate the modulatory effect of Nigella sativa (NS) oil on the selected T cell subset percentage in females with RA.
A randomized, double-blinded placebo-controlled, 2 months, parallel-group clinical trial was conducted. Forty-three female patients (20-50 years) with mild to moderate RA were recruited and assigned into NS (n = 23) and placebo (n = 20) groups to receive one gram of NS oil, or starch, capsule in two divided doses, respectively. The disease activity scores of 28 joints (DAS28) were calculated and percentages of CD4(+), CD8(+), and CD4(+)CD25(+) T cells were examined using flow cytometry.
Treatment with NS led to significant reduction of the serum high-sensitivity C-reactive protein (hs-CRP) level and DAS-28 score and an improved number of swollen joints compared with baseline and placebo groups. A relatively comparable CD4(+) T cell percentage was observed in the NS and placebo groups either in baseline or the end of study. The treatment also resulted in reduced CD8(+), and increased CD4(+)CD25(+) T cell percentage and the CD4(+)/CD8(+) ratio as compared to placebo and baseline. A negative significant correlation between changes in CD8(+) and changes in CD4(+)CD25(+) T cells and a positive significant correlation between changes in CD4(+)CD25(+) T cells and changes in the CD4(+)/CD8(+) ratio was observed in the NS group.
This study gives strength to the potential relevance of NS in clinical management of RA through modulation of T lymphocytes.
大量证据表明，CD4(+)、CD8(+)和CD4(+)CD25(+) T淋巴细胞参与类风湿关节炎（RA）的诱导和/或保护过程。我们旨在研究黑种草（NS）油对RA女性患者中选定T细胞亚群百分比的调节作用。
进行了一项为期2个月的随机、双盲、安慰剂对照平行组临床试验。招募了43名年龄在20至50岁之间的轻度至中度RA女性患者，分为NS组（n = 23）和安慰剂组（n = 20），分别接受1克NS油或淀粉胶囊，分两次服用。计算28个关节的疾病活动评分（DAS28），并使用流式细胞术检测CD4(+)、CD8(+)和CD4(+)CD25(+) T细胞的百分比。
与基线和安慰剂组相比，NS治疗导致血清高敏C反应蛋白（hs-CRP）水平和DAS-28评分显著降低，肿胀关节数量减少。在基线或研究结束时，NS组和安慰剂组的CD4(+) T细胞百分比相对相当。与安慰剂组和基线相比，该治疗还导致CD8(+) T细胞百分比降低，CD4(+)CD25(+) T细胞百分比和CD4(+)/CD8(+)比值升高。在NS组中，观察到CD8(+) T细胞变化与CD4(+)CD25(+) T细胞变化之间存在显著负相关，CD4(+)CD25(+) T细胞变化与CD4(+)/CD8(+)比值变化之间存在显著正相关。
本研究证实了NS通过调节T淋巴细胞在RA临床管理中的潜在相关性。
