可生物降解聚合物涂层对Mg-2.0Zn-0.98Mn镁合金的腐蚀、细胞相容性和细胞功能的影响
Influence of biodegradable polymer coatings on corrosion, cytocompatibility and cell functionality of Mg-2.0Zn-0.98Mn magnesium alloy.
作者信息
Witecka Agnieszka, Yamamoto Akiko, Idaszek Joanna, Chlanda Adrian, Święszkowski Wojciech
机构信息
International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), Tsukuba, Ibaraki 305-0044, Japan; Faculty of Materials Science and Engineering, Warsaw University of Technology (WUT), 141 Woloska Street, Warsaw 02-507, Poland.
International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), Tsukuba, Ibaraki 305-0044, Japan.
出版信息
Colloids Surf B Biointerfaces. 2016 Aug 1;144:284-292. doi: 10.1016/j.colsurfb.2016.04.021. Epub 2016 Apr 11.
Four kinds of biodegradable polymers were employed to prepare bioresorbable coatings on Mg-2.0Zn-0.98Mn (ZM21) alloy to understand the relationship between polymer characteristics, protective effects on substrate corrosion, cytocompatibility and cell functionality. Poly-l-lactide (PLLA), poly(3-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) or poly(lactic-co-glycolic) acid (PLGA) was spin-coated on ZM21, obtaining a smooth, non-porous coating less than 0.5μm in thickness. Polymer coating characterization, a degradation study, and biocompatibility evaluations were performed. After 4 w of immersion into cell culture medium, degradation of PLGA and PLLA coatings were confirmed by ATR-FTIR observation. The coatings of PLLA, PHB and PHBV, which have lower water permeability and slower degradation than PLGA, provide better suppression of initial ZM21 degradation and faster promotion of human osteosarcoma cell growth and differentiation.
采用四种可生物降解聚合物在Mg-2.0Zn-0.98Mn（ZM21）合金上制备可生物吸收涂层，以了解聚合物特性、对基体腐蚀的保护作用、细胞相容性和细胞功能之间的关系。将聚左旋乳酸（PLLA）、聚3-羟基丁酸酯（PHB）、聚3-羟基丁酸酯-co-3-羟基戊酸酯（PHBV）或聚乳酸-乙醇酸共聚物（PLGA）旋涂在ZM21上，得到厚度小于0.5μm的光滑无孔涂层。进行了聚合物涂层表征、降解研究和生物相容性评估。在浸入细胞培养基4周后，通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）观察证实了PLGA和PLLA涂层的降解。与PLGA相比，PLLA、PHB和PHBV涂层具有较低的水渗透性和较慢的降解速度，能更好地抑制ZM21的初始降解，并更快地促进人骨肉瘤细胞的生长和分化。
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