裂谷热的快速诊断：人血清中病毒抗原直接检测方法的比较
Rapid diagnosis of Rift Valley fever: a comparison of methods for the direct detection of viral antigen in human sera.
作者信息
Meegan J, Le Guenno B, Ksiazek T, Jouan A, Knauert F, Digoutte J P, Peters C J
机构信息
U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21701.
出版信息
Res Virol. 1989 Jan-Feb;140(1):59-65. doi: 10.1016/s0923-2516(89)80085-8.
Human sera collected during the 1987 Rift Valley fever (RVF) epidemic in the Senegal River basin were analysed using three enzyme immunoassays to establish the best method for rapid diagnosis of RVF. A biotin-avidin-enhanced antigen detection method utilizing monoclonal antibodies proved most sensitive. Eighty-two viremic human sera were tested, and this assay detected antigen in 29.3% of the samples.
对1987年在塞内加尔河流域发生裂谷热（RVF）疫情期间采集的人类血清，采用三种酶免疫测定法进行分析，以确定快速诊断RVF的最佳方法。一种利用单克隆抗体的生物素-抗生物素蛋白增强抗原检测方法被证明最为灵敏。对82份病毒血症人类血清进行了检测，该测定法在29.3%的样本中检测到了抗原。
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