Santiago Alba, Pozuelo Marta, Poca Maria, Gely Cristina, Nieto Juan Camilo, Torras Xavier, Román Eva, Campos David, Sarrabayrouse Guillaume, Vidal Silvia, Alvarado-Tapias Edilmar, Guarner Francisco, Soriano German, Manichanh Chaysavanh, Guarner Carlos
Department of Gastroenterology, Vall d'Hebron Research Institute, Passeig Vall d'Hebron 119-129, Barcelona 08035, Spain.
Department of Gastroenterology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
Sci Rep. 2016 Apr 26;6:25001. doi: 10.1038/srep25001.
The progression of cirrhosis is associated with alterations in the composition of the gut microbiome. To assess microbial translocation, we compared the serum microbial composition of patients with and without ascites and characterized the ascitic fluid microbiome using 16S rDNA high-throughput sequencing data. A complex and specific microbial community was detected in the serum and ascitic fluid of patients with cirrhosis but barely detectable in the serum of healthy controls. The serum microbiome of patients with ascites presented higher levels of lipopolysaccharide binding protein, a marker of microbial translocation, associated with higher diversity and relative abundance of Clostridiales and an unknown genus belonging to the Cyanobacteria phylum compared to patients without ascites. The composition of the fecal microbiome was also more altered in patients with than without ascites, confirming previous studies on fecal microbiome. We propose that alteration of the serum and fecal microbiome composition be considered indicators of cirrhosis progression.
肝硬化的进展与肠道微生物群组成的改变有关。为了评估微生物易位，我们比较了有腹水和无腹水患者的血清微生物组成，并使用16S rDNA高通量测序数据对腹水微生物群进行了特征分析。在肝硬化患者的血清和腹水中检测到了一个复杂且特定的微生物群落，但在健康对照者的血清中几乎检测不到。与无腹水患者相比，有腹水患者的血清微生物群呈现出更高水平的脂多糖结合蛋白，这是微生物易位的一个标志物，与梭菌目以及属于蓝细菌门的一个未知属的更高多样性和相对丰度相关。有腹水患者的粪便微生物群组成也比无腹水患者改变得更多，这证实了先前关于粪便微生物群的研究。我们建议将血清和粪便微生物群组成的改变视为肝硬化进展的指标。
