Goodarzi Golnaz, Qujeq Durdi, Elmi Maryam M, Feizi Farideh, Fathai Sadegh
Cellular and Molecular Biology Research Center (CMBRC), Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Department of Clinical Biochemistry, Babol University of Medical Sciences, Babol, Iran.
Cell Biochem Funct. 2016 Jun;34(4):242-9. doi: 10.1002/cbf.3186. Epub 2016 Apr 25.
Diabetes is now regarded as a major public health problem. The number of patients is estimated to increase to over 439 million cases by 2030. One of the major health clinical problems in patients with diabetes patients is impaired wound healing. Diabetic foot ulcer is a major complication of diabetes mellitus in 12 to 25% of patients, which increases the risk of damage in the limbs or amputation. The earthworm Eisenia foetida glycolipoprotein (as known G-90) is a blend of macromolecules with some biological properties including mitogenicity, anticoagulation, fibrinolysis, bacteriostatic and antioxidatiaon. Given the biological properties of G-90, this study was conducted to investigate the effect of extract obtained from the homogenate of Eisenia foetida (G-90) on the wound healing process in alloxan-induced diabetic rats. The results of the present study revealed that treatment by using G-90 can speed up the wound healing process, which is exactly similar to the effect of D-panthenol treatment in rats. These findings also demonstrated that G-90 treatment decreases the risk of infection in the wound site compared with D-panthenol treatment. In addition, histological analysis indicated that a better extracellular matrix formation with increased fibroblast proliferation, neovascularization, collagen synthesis and early epithelial layer formation was observed in G-90 treated group. Therefore, the G-90 could be considered as a new wound healing agent introducing promising therapeutic approaches in both human and veterinary medicine. Copyright © 2016 John Wiley & Sons, Ltd.
糖尿病如今被视为一个重大的公共卫生问题。据估计，到2030年患者数量将增至超过4.39亿例。糖尿病患者的主要健康临床问题之一是伤口愈合受损。糖尿病足溃疡是12%至25%糖尿病患者的主要并发症，会增加肢体损伤或截肢的风险。蚯蚓赤子爱胜蚓糖脂蛋白（即G - 90）是一种具有多种生物学特性的大分子混合物，包括促有丝分裂、抗凝、纤维蛋白溶解、抑菌和抗氧化作用。鉴于G - 90的生物学特性，本研究旨在探讨从赤子爱胜蚓匀浆中提取的提取物（G - 90）对四氧嘧啶诱导的糖尿病大鼠伤口愈合过程的影响。本研究结果显示，使用G - 90进行治疗可加速伤口愈合过程，这与大鼠中D - 泛醇治疗的效果完全相似。这些发现还表明，与D - 泛醇治疗相比，G - 90治疗可降低伤口部位的感染风险。此外，组织学分析表明，在G - 90治疗组中观察到细胞外基质形成更好，成纤维细胞增殖、新血管形成、胶原蛋白合成增加以及早期上皮层形成。因此，G - 90可被视为一种新型伤口愈合剂，在人类和兽医学中引入了有前景的治疗方法。版权所有© 2016约翰威立父子有限公司。
