Khan Aftab Aslam Parwaz, Khan Anish, Rahman Mohammed M, Asiri Abdullah M, Oves Mohammad
Center of Excellence for Advanced Materials Research and Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, P.O. Box 80203, Saudi Arabia.
Center of Excellence for Advanced Materials Research and Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, P.O. Box 80203, Saudi Arabia.
Int J Biol Macromol. 2016 Aug;89:198-205. doi: 10.1016/j.ijbiomac.2016.04.064. Epub 2016 Apr 22.
Graphene oxide/carbon nanotube/poly (O-toluidine) (GO-CNT-POT) nanocomposite was prepared by a situ polymerization method and characterized by X-ray powder diffractometry (XRD) and scanning electron microscopy (SEM). The antibacterial activity of the obtained GO-CNT-POT nanocomposite was also evaluated against Gram positive bacteria Bacillus subtilis, Gram negative bacteria Escherichia coli and antibiotics (Amoxicillin) using the agar plate. The antibacterial study showed that the GO-CNT-POT was found to be most effective against both B. subtilis and E. coli respectively which was significant compared to the amoxicillin and the simultaneously GO-CNT-POT nanocomposite were fabricated onto glassy carbon electrode (GCE) using conducting coating binders by I-V technique, where the total analytical parameters were measured for the development of sensitive lead sensors (Pb(2+)). The GO-CNT-POT nanocomposite were deposited on flat-GCE (surface area: ∼0.0316cm(2)) to result in a sensor that has a fast response to selective Pb(2+) ions in buffer system. Features including sensitivity, detection limit, reproducibility, linear dynamic range, selectivity, and electrochemical performances were investigated in details with the GO-CNT-POT nanocomposite fabricated GCE electrodes. The calibration plot is linear (r(2): 0.9907) over the large concentration range (0.1nM to 1.0mM). The sensitivity and detection limit is calculated as 8.53164μAcm(-2)μM(-1) and 89.0 pM (at a signal-to-noise-ratio, SNR of 3) respectively.
采用原位聚合法制备了氧化石墨烯/碳纳米管/聚邻甲苯胺（GO-CNT-POT）纳米复合材料，并通过X射线粉末衍射仪（XRD）和扫描电子显微镜（SEM）对其进行了表征。还使用琼脂平板法评估了所得GO-CNT-POT纳米复合材料对革兰氏阳性菌枯草芽孢杆菌、革兰氏阴性菌大肠杆菌以及抗生素（阿莫西林）的抗菌活性。抗菌研究表明，GO-CNT-POT分别对枯草芽孢杆菌和大肠杆菌最有效，与阿莫西林相比具有显著差异，同时采用I-V技术使用导电涂层粘合剂将GO-CNT-POT纳米复合材料制备在玻碳电极（GCE）上，在此处测量了用于开发灵敏铅传感器（Pb(2+)）的总分析参数。将GO-CNT-POT纳米复合材料沉积在平面GCE（表面积：~0.0316cm(2)）上，得到一种对缓冲体系中选择性Pb(2+)离子具有快速响应的传感器。使用GO-CNT-POT纳米复合材料制备的GCE电极详细研究了包括灵敏度、检测限、重现性、线性动态范围、选择性和电化学性能等特征。校准曲线在较大浓度范围（0.1nM至1.0mM）内呈线性（r(2)：0.9907）。灵敏度和检测限分别计算为8.53164μAcm(-2)μM(-1)和89.0 pM（在信噪比为3时）。
