Song Hai, Xu Lincheng, Zhang Rongping, Cao Zhenzhen, Zhang Huan, Yang Li, Guo Zeyun, Qu Yongqiang, Yu Jianyun
Department of Forensic Medicine, Kunming Medical University, Kunming, Yunnan 650500, China; Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China.
Department of Forensic Medicine, Kunming Medical University, Kunming, Yunnan 650500, China.
Neurosci Lett. 2016 May 27;622:95-101. doi: 10.1016/j.neulet.2016.04.048. Epub 2016 Apr 22.
In this study, we investigated whether Rosemary extract (RE) improved cognitive deficits in repetitive mild Traumatic brain injury (rmTBI) rats and its potential mechanisms. The present results showed that rmTBI caused cognitive deficits, such as increased latency to find platform and decreased time spent in target quadrant in Morris water maze (MWM). These behavioral alterations were accompanying with the increased neuronal degeneration and glial fibrillary acidic protein (GFAP)-positive cells, increased Reactive oxygen species (ROS) generation, decreased activity of Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx) and Catalase (CAT), elevated protein level of IL-1β, IL-6 and TNF-α in hippocampus. Treatment with RE prevented these changes above. Our findings confirmed the effect of rosemary extract on improvement of cognitive deficits and suggested its mechanisms might be mediated by anti-oxidative and anti-inflammatory. Therefore, rosemary extract may be a potential treatment to improve cognitive deficits in rmTBI patients.
在本研究中，我们探究了迷迭香提取物（RE）是否能改善重复性轻度创伤性脑损伤（rmTBI）大鼠的认知缺陷及其潜在机制。目前的结果表明，rmTBI导致了认知缺陷，如在莫里斯水迷宫（MWM）中找到平台的潜伏期增加以及在目标象限停留的时间减少。这些行为改变伴随着神经元变性增加、胶质纤维酸性蛋白（GFAP）阳性细胞增多、活性氧（ROS）生成增加、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）活性降低、海马中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）蛋白水平升高。用RE治疗可防止上述这些变化。我们的研究结果证实了迷迭香提取物对改善认知缺陷的作用，并表明其机制可能是通过抗氧化和抗炎介导的。因此，迷迭香提取物可能是改善rmTBI患者认知缺陷的一种潜在治疗方法。
