Martins Conceição S, Leitão Renata F C, Costa Deiziane V S, Melo Iracema M, Santos Glaylton S, Lima Vilma, Baldim Victor, Wong Deysi V T, Bonfim Luana E, Melo Cíntia B, G de Oliveira Marcelo, Brito Gerly A C
Postgraduate Program in Morphofunctional Sciences, Department of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Department of Clinical Dentistry, Faculty of Pharmacy, Dentistry and Nursing, School of Dentistry. Federal University of Ceará, Fortaleza, Ceará, Brazil.
PLoS One. 2016 Apr 26;11(4):e0153716. doi: 10.1371/journal.pone.0153716. eCollection 2016.
S-nitrosoglutathione (GSNO) is a nitric oxide (NO) donor, which exerts antioxidant, anti-inflammatory, and microbicidal actions. Intragingival application of GSNO was already shown to decrease alveolar bone loss, inflammation and oxidative stress in an experimental periodontal disease (EPD) model. In the present study, we evaluated the potential therapeutic effect of topical applications of hydroxypropylmethylcellulose (HPMC)/GSNO solutions on EPD in Wistar rats. EPD was induced by placing a sterilized nylon (3.0) thread ligature around the cervix of the second left upper molar of the animals, which received topical applications of a HPMC solutions containing GSNO 2 or 10 mM or vehicle (HPMC solution), 1 h prior to the placement of the ligature and then twice daily until sacrifice on day 11. Treatment with HPMC/GSNO 10 mM solution significantly reduced alveolar bone loss, oxidative stress and TNF-α e IL-1β levels in the surrounding gingival tissue, and led to a decreased transcription of RANK and TNF-α genes and elevated bone alkaline phosphatase, compared to the HPMC group. In conclusion, topical application of HPMC/GSNO solution is a potential treatment to reduce inflammation and bone loss in periodontal disease.
S-亚硝基谷胱甘肽（GSNO）是一种一氧化氮（NO）供体，具有抗氧化、抗炎和杀菌作用。在实验性牙周病（EPD）模型中，龈内应用GSNO已被证明可减少牙槽骨吸收、炎症和氧化应激。在本研究中，我们评估了局部应用羟丙基甲基纤维素（HPMC）/GSNO溶液对Wistar大鼠EPD的潜在治疗效果。通过在动物左上颌第二磨牙颈部放置消毒尼龙（3.0）丝线结扎来诱导EPD，在结扎前1小时，对动物局部应用含有2 mM或10 mM GSNO的HPMC溶液或赋形剂（HPMC溶液），然后每天两次，直至在第11天处死。与HPMC组相比，用10 mM HPMC/GSNO溶液治疗可显著减少牙槽骨吸收、氧化应激以及周围牙龈组织中TNF-α和IL-1β水平，并导致RANK和TNF-α基因转录减少以及骨碱性磷酸酶升高。总之，局部应用HPMC/GSNO溶液是减少牙周病炎症和骨吸收的一种潜在治疗方法。
