Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore.
Chem Soc Rev. 2016 Aug 7;45(15):4199-225. doi: 10.1039/c5cs00700c. Epub 2016 Apr 27.
Since many bionanotechnologies are targeted at cells, understanding how and where their interactions occur and the subsequent results of these interactions is important. Changing the intrinsic properties of DNA nanostructures and linking them with interactions presents a holistic and powerful strategy for understanding dual nanostructure-biological systems. With the recent advances in DNA nanotechnology, DNA nanostructures present a great opportunity to understand the often convoluted mass of information pertaining to nanoparticle-biological interactions due to the more precise control over their chemistry, sizes, and shapes. Coupling just some of these designs with an understanding of biological processes is both a challenge and a source of opportunities. Despite continuous advances in the field of DNA nanotechnology, the intracellular fate of DNA nanostructures has remained unclear and controversial. Because understanding its cellular processing and destiny is a necessary prelude to any rational design of exciting and innovative bionanotechnology, in this review, we will discuss and provide a comprehensive picture relevant to the intracellular processing and the fate of various DNA nanostructures which have been remained elusive for some time. We will also link the unique capabilities of DNA to some novel ideas for developing next-generation bionanotechnologies.
由于许多生物纳米技术针对的是细胞，因此了解它们的相互作用发生的方式和位置以及这些相互作用的后续结果非常重要。改变 DNA 纳米结构的固有性质并将它们与相互作用联系起来，为理解双纳米结构-生物系统提供了一种整体而强大的策略。随着 DNA 纳米技术的最新进展，由于对其化学性质、大小和形状有更精确的控制，DNA 纳米结构为理解纳米颗粒-生物相互作用中常常错综复杂的大量信息提供了很好的机会。将这些设计中的一些与对生物过程的理解相结合，既是一个挑战，也是一个机会的来源。尽管 DNA 纳米技术领域不断取得进展，但 DNA 纳米结构在细胞内的命运仍不清楚且存在争议。因为了解其细胞处理和命运是任何激动人心的创新生物纳米技术合理设计的必要前提，在这篇综述中，我们将讨论并提供与各种 DNA 纳米结构的细胞内处理和命运相关的全面情况，这些结构在一段时间以来一直难以捉摸。我们还将把 DNA 的独特功能与一些开发下一代生物纳米技术的新想法联系起来。
