Zhao Yixiu, Gao Feng, Zhang Yong, Wang Hongtao, Zhu Jiuxin, Chang Liping, Du Zhimin, Zhang Yan
Department of Pharmacology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
State Key Laboratory of Collateral Disease Research and Innovation Medicine, Hebei Yiling Pharmaceutical Research Institute, Shijiazhuang, Hebei 050000, P.R. China.
Mol Med Rep. 2016 Jun;13(6):5185-92. doi: 10.3892/mmr.2016.5203. Epub 2016 Apr 28.
Shensong Yangxin capsules (SSYX) are an effective traditional Chinese medicine that has been used to treat coronary heart disease clinically. The present study aimed to establish whether SSYX prevent ischemic arrhythmias in rats, and to explore the underlying mechanisms. Male rats were pretreated with distilled water, SSYX and amiodarone for one week. Acute myocardial ischemia (AMI) was performed to induce ischemic arrhythmias. The incidence and severity of ischemic arrhythmias were evaluated. The action potential, transient outward K+ current (Ito) and inward rectifier K+ current (IK1) of rat cardiomyocytes were measured using the patch‑clamp technique. The intracellular Ca2+ concentration of the cardiomyocytes was measured using a laser scanning confocal microscope. The results revealed that SSYX lowered the incidence of arrhythmia markedly during AMI. Furthermore, SSYX delayed the appearance, and reduced the severity, of ischemic arrhythmias compared with the control. In addition, SSYX markedly decreased the ratio of the myocardial infarction region to the whole heart. In an in vitro study, SSYX prolonged the action potential duration of rat cardiomyocytes, and inhibited Ito and IK1 markedly. Additionally, SSYX inhibited Ca2+ elevation induced by KCl in cardiomyocytes. These results suggested that SSYX prevents ischemic arrhythmia, and the underlying mechanism responsible for this process may include prolonging the action potential and alleviating Ca2+ overload.
参松养心胶囊（SSYX）是一种有效的传统中药，临床上已用于治疗冠心病。本研究旨在确定参松养心胶囊是否能预防大鼠缺血性心律失常，并探讨其潜在机制。雄性大鼠分别用蒸馏水、参松养心胶囊和胺碘酮预处理一周。进行急性心肌缺血（AMI）以诱导缺血性心律失常。评估缺血性心律失常的发生率和严重程度。采用膜片钳技术测量大鼠心肌细胞的动作电位、瞬时外向钾电流（Ito）和内向整流钾电流（IK1）。使用激光扫描共聚焦显微镜测量心肌细胞内的Ca2+浓度。结果显示，参松养心胶囊在急性心肌缺血期间显著降低了心律失常的发生率。此外，与对照组相比，参松养心胶囊延迟了缺血性心律失常的出现，并降低了其严重程度。此外，参松养心胶囊显著降低了心肌梗死面积与全心面积的比值。在体外研究中，参松养心胶囊延长了大鼠心肌细胞的动作电位时程，并显著抑制了Ito和IK1。此外，参松养心胶囊抑制了氯化钾诱导的心肌细胞Ca2+升高。这些结果表明，参松养心胶囊可预防缺血性心律失常，其潜在机制可能包括延长动作电位和减轻Ca2+超载。
