Ni Junjun, Xu Li, Li Wei, Wu Lijun
Beijing Harmony Health Medical Diagnostics Co. Ltd, Beijing, China.
Department of Medicinal Chemistry in Harbin Medical University, Harbin, China.
Biomed Chromatogr. 2016 Nov;30(11):1796-1806. doi: 10.1002/bmc.3755. Epub 2016 Jun 8.
Easy-to-use early cancer detection methods based on metabolomics using serum samples have been developed recently. Among metabolites, amino acids and acylcarnitine are two of the most suitable candidates for diagnosing lung cancer. The purpose of the present study was to develop a novel, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to simultaneously determine 13 amino acids and 8 acylcarnitines in lung cancer patients in serum. After derivatization, the 21 analytes were separated using a C column with gradient elution program in 14 min, obtaining recovery within 90.4-113.8% and precision within 0.3-14.8%. The method was successfully applied in concentration determination of lung cancer patients and healthy controls. The results showed that the serum concentration of lung cancer patients were significant from those of healthy controls.
近年来，基于代谢组学并使用血清样本的易于使用的早期癌症检测方法已被开发出来。在代谢物中，氨基酸和酰基肉碱是诊断肺癌最合适的两个候选物。本研究的目的是开发一种新颖、灵敏且特异的液相色谱-串联质谱（LC-MS/MS）方法，以同时测定肺癌患者血清中的13种氨基酸和8种酰基肉碱。衍生化后，使用C柱通过梯度洗脱程序在14分钟内分离出21种分析物，回收率在90.4%-113.8%之间，精密度在0.3%-14.8%之间。该方法成功应用于肺癌患者和健康对照的浓度测定。结果表明，肺癌患者的血清浓度与健康对照者有显著差异。
