Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.
BIOMIC_Auth, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center B.1.4, 10th km Thessaloniki-Thermi Rd, P.O. Box 8318, 57001, Thessaloniki, Greece.
Anal Bioanal Chem. 2022 Apr;414(9):3095-3108. doi: 10.1007/s00216-022-03940-9. Epub 2022 Feb 17.
Acylcarnitines are essential diagnostic markers for complex diseases and fatty acid metabolism disorders, and play an important role in cardiovascular diseases. Herein, a HILIC-MS/MS method was developed and validated for the rapid quantitation of the acylcarnitines C2, C3, C4, C5, C6, C8, C10, C12, C14, C16, C18, C18:1 and C18:2 in human serum. RPLC and HILIC modes were tested, and HILIC was selected since it provided optimum analyte separation. Intra- and interday accuracy ranged from 90.4% to 114% and from 96% to 112%, respectively, while intra- and interday precision ranged from 0.37% to 13.7% and from 1.3% to 9.5%, respectively. A limit of quantitation (LOQ) of 78.1 ng/mL was found for C2, 2.4 ng/mL for C3, C18:1 and C18:2, and 1.2 ng/mL for C4, C5, C6, C8, C10, C12, C14, C16, and C18. Method validation was performed in accordance with bioanalytical method guidelines. Subsequently the method was applied in the analysis of approximately 1040 samples from patients with coronary artery disease.
酰基肉碱是复杂疾病和脂肪酸代谢紊乱的重要诊断标志物，在心血管疾病中发挥着重要作用。本文建立并验证了一种用于快速定量检测人血清中酰基肉碱 C2、C3、C4、C5、C6、C8、C10、C12、C14、C16、C18、C18:1 和 C18:2 的亲水作用色谱-串联质谱（HILIC-MS/MS）方法。我们测试了反相液相色谱（RPLC）和亲水作用色谱（HILIC）模式，选择了 HILIC 模式，因为它可以提供最佳的分析物分离效果。日内和日间准确度的范围分别为 90.4%至 114%和 96%至 112%，而日内和日间精密度的范围分别为 0.37%至 13.7%和 1.3%至 9.5%。我们发现 C2 的定量下限（LOQ）为 78.1ng/mL，C3、C18:1 和 C18:2 的 LOQ 为 2.4ng/mL，C4、C5、C6、C8、C10、C12、C14、C16 和 C18 的 LOQ 为 1.2ng/mL。方法验证符合生物分析方法指南的要求。随后，我们应用该方法分析了大约 1040 例冠状动脉疾病患者的样本。
