Witzler Markus, Küllmer Fabian, Hirtz Annika, Günther Klaus
Institute of Nutritional and Food Sciences, Food Chemistry, Rheinische Friedrich-Wilhelms-Universität Bonn , Endenicher Allee 11-13, 53115 Bonn, Germany.
J Agric Food Chem. 2016 May 25;64(20):4165-70. doi: 10.1021/acs.jafc.6b01248. Epub 2016 May 12.
With the increasing use of nanoparticles in consumer products, the need for validated quantitation methods also rises. This becomes even more urgent because the risks of nanomaterials are still not conclusively assessed. Fast, accurate, and robust single-particle (sp) ICP-MS is a promising technique as it is capable of counting and sizing particles at very low concentrations at the same time. Another feature is the simultaneous distinction between dissolved and particulate analytes. The present study shows, for the first time to our knowledge, a method validation for the rapid analysis of silver and gold nanoparticles with sp-ICP-MS in fruit juices without sample preparation. The investigated matrices water, orange juice, and apple juice were spiked with particles and only diluted prior to measurement without using a digestion reagent. The validations regarding particle size are successful according to the German GTFCh's guideline with deviations of accuracy and precision below 15%.
随着纳米颗粒在消费品中的使用日益增加，对经过验证的定量方法的需求也随之上升。由于纳米材料的风险仍未得到最终评估，这一需求变得更加迫切。快速、准确且稳健的单颗粒（sp）电感耦合等离子体质谱法是一种很有前景的技术，因为它能够同时对极低浓度的颗粒进行计数和尺寸测定。另一个特点是能够同时区分溶解态和颗粒态分析物。据我们所知，本研究首次展示了一种无需样品前处理，用sp-ICP-MS快速分析果汁中银和金纳米颗粒的方法验证。所研究的基质水、橙汁和苹果汁加入颗粒后，在测量前仅进行稀释，不使用消解试剂。根据德国GTFCh的指南，关于粒径的验证是成功的，准确度和精密度偏差低于15%。
