人类和小鼠组织中抗原呈递细胞的鉴定与分离方案。

Protocols for the Identification and Isolation of Antigen-Presenting Cells in Human and Mouse Tissues.

作者信息

McGovern Naomi, Schlitzer Andreas, Janela Baptiste, Ginhoux Florent

机构信息

Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, IMMUNOS Building #3-4, Biopolis, Singapore, 138648, Singapore.

出版信息

Methods Mol Biol. 2016;1423:169-80. doi: 10.1007/978-1-4939-3606-9_12.

DOI:10.1007/978-1-4939-3606-9_12

PMID:27142016

Abstract

The efficient processing of both mouse and human tissues is a valuable technique for characterizing tissue-associated immune cells. Here, we describe the techniques used and optimised within our laboratory for the enrichment and identification of antigen-presenting cells across a number of mouse and human tissues.

摘要

对小鼠和人类组织进行高效处理是表征组织相关免疫细胞的一项重要技术。在此，我们描述了在我们实验室中用于富集和鉴定多种小鼠和人类组织中抗原呈递细胞的技术及其优化方法。