Han Mei, Xu Zhi-Yuan, Du Chao, Qian He, Zhang Wei-Guo
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, 1800# Lihu Road, Wuxi, 214122, JiangSu, China.
State Key Laboratory of Dairy Biotechnology, Technology Center Bright Dairy and Food Co., Ltd, 200436, Shanghai, China.
Bioprocess Biosyst Eng. 2016 Sep;39(9):1425-33. doi: 10.1007/s00449-016-1620-y. Epub 2016 May 4.
Nitrogen limited but carbon excess condition was used to obtain high cellular lipid content and production. The maximum lipid production was 51 g/L, the lipid content in the dry cell was 60 %, and the lipid productivity was 0.53 g/L/h. In the fermentation, the content of lipid was raised from 20 % of dry cell weight to 60 %, and the proportion of oleic acid was raised from 66.8 to 72.5 %. Meanwhile, the metabolism of carotenoids switched to torulene, and its proportion was raised from 30 to 58 %. This was according to torulene had the better antioxidant ability than β-carotene to protect the strain from oxidative damage proved by their ABTS* radical scavenging activity and lipid peroxidation inhibition ability. Sporidiobolus pararoseus lipid was a good source of lipid not only because of its high oleic acid composition, but also the antioxidant ability of carotenoids in the lipid.
采用氮限制但碳过量的条件来获得高细胞脂质含量和产量。最大脂质产量为51 g/L，干细胞中的脂质含量为60%，脂质生产率为0.53 g/L/h。在发酵过程中，脂质含量从干细胞重量的20%提高到60%，油酸比例从66.8%提高到72.5%。同时，类胡萝卜素的代谢转变为玫瑰孢菌素，其比例从30%提高到58%。这是因为通过ABTS*自由基清除活性和脂质过氧化抑制能力证明，玫瑰孢菌素比β-胡萝卜素具有更好的抗氧化能力，能保护菌株免受氧化损伤。副玫瑰掷孢酵母脂质不仅因其高油酸组成，还因其脂质中类胡萝卜素的抗氧化能力，是一种优质的脂质来源。
