Liu Chang, Cheng Yuliang, Du Chao, Lv Tianqi, Guo Yahui, Han Mei, Pi Fuwei, Zhang Weiguo, Qian He
a State Key Laboratory of Food Science and Technology, Jiangnan University , Wuxi , China.
b Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University , Wuxi , China.
Prep Biochem Biotechnol. 2019;49(8):767-774. doi: 10.1080/10826068.2019.1608448. Epub 2019 May 3.
The cell wall of carotenoids producing yeast was broken through five different methods: acid-heating method, dimethyl sulfoxide (DMSO) method, enzymatic method, high-pressure homogenization (HPH) method, and cell autolysis method. HPH method not only brought the optimum breaking effect (wall-breaking extent of 72.3%) and the highest carotenoid extraction rate (67.2%), but also had the advantages of short-time, simple process, safe, and pollution-free. After optimization, the wall-breaking extent and the carotenoid extraction rate were enhanced to 78.3% and 82.5%, respectively. And the optimum conditions of HPH were obtained as homogenization pressure 80 MPa, bacterial liquid concentration 8% and homogenization for three times. Moreover, cell experiments demonstrated that all of the four carotenoids (β-carotene, γ-carotene, torulene, and torularhodin) purified from intracellular products of had the effect of resistance to oxidative damage from hydrogen peroxide on SK-HEP-1 cells, and torulene showed the most notable effect among them.
酸热法、二甲基亚砜（DMSO）法、酶解法、高压均质（HPH）法和细胞自溶法。HPH法不仅带来了最佳的破壁效果（破壁率达72.3%）和最高的类胡萝卜素提取率（67.2%），而且具有时间短、工艺简单、安全且无污染的优点。优化后，破壁率和类胡萝卜素提取率分别提高到78.3%和82.5%。HPH的最佳条件为均质压力80MPa、菌液浓度8%且均质三次。此外，细胞实验表明，从其细胞内产物中纯化得到的四种类胡萝卜素（β-胡萝卜素、γ-胡萝卜素、圆酵母素和红酵母红素）均对SK-HEP-1细胞具有抵抗过氧化氢氧化损伤的作用，其中圆酵母素的效果最为显著。
