Wojnar Weronika, Zych Maria, Borymski Sławomir, Kaczmarczyk-Sedlak Ilona
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Katowice, Jagiellońska 4, 41-200 Sosnowiec, Poland.
Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland.
Antioxidants (Basel). 2020 Feb 16;9(2):160. doi: 10.3390/antiox9020160.
Prolonged hyperglycemia is one of the main causes of reactive oxygen species and free radicals generation in diabetes which may affect various organs, including the eye. Oxidative damage to proteins and lipids in the eye lens could lead to cataract formation. To cope with oxidative stress, the endogenous antioxidative system may be supported by the supplementation of exogenous antioxidants. The aim of this study was to evaluate the effect of chrysin, a natural flavonoid, on oxidative stress and polyol pathway-related markers in the lenses of streptozotocin-induced type 1 male diabetic rats. Chrysin at doses of 50 and 100 mg/kg was administered by gavage for 28 days. This treatment resulted in a decrease in antioxidative enzymes activity and oxidative stress index. Moreover, chrysin administration elevated the reduced glutathione level in the lenses. A decrease in the markers linked to oxidative damage to proteins and lipids in the lenses was noted, especially after treatment with 50 mg/kg of chrysin. Neither of the chrysin doses affected glycemia-related markers in the serum or altered parameters related to the polyol pathway and advanced glycation end-products level in the lenses of diabetic rats. Upon obtaining results, it can be concluded that chrysin reveals antioxidative activity in the lenses but shows no antihyperglycemic or antiglycation properties.
长期高血糖是糖尿病中活性氧和自由基产生的主要原因之一，这可能会影响包括眼睛在内的各种器官。晶状体中蛋白质和脂质的氧化损伤可能导致白内障形成。为了应对氧化应激，内源性抗氧化系统可能需要外源性抗氧化剂的补充来支持。本研究的目的是评估天然黄酮白杨素对链脲佐菌素诱导的1型雄性糖尿病大鼠晶状体氧化应激和多元醇途径相关标志物的影响。以50和100mg/kg的剂量通过灌胃给予白杨素，持续28天。这种治疗导致抗氧化酶活性和氧化应激指数降低。此外，给予白杨素提高了晶状体中还原型谷胱甘肽的水平。注意到与晶状体中蛋白质和脂质氧化损伤相关的标志物减少，尤其是在用50mg/kg白杨素治疗后。两种白杨素剂量均未影响血清中与血糖相关的标志物，也未改变糖尿病大鼠晶状体中与多元醇途径和晚期糖基化终产物水平相关的参数。根据结果可以得出结论，白杨素在晶状体中具有抗氧化活性，但没有抗高血糖或抗糖化特性。
