南非产MCR-1的耐黏菌素大肠杆菌分离株的遗传特征
Genetic Features of MCR-1-Producing Colistin-Resistant Escherichia coli Isolates in South Africa.
作者信息
Poirel Laurent, Kieffer Nicolas, Brink Adrian, Coetze Jennifer, Jayol Aurélie, Nordmann Patrice
机构信息
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland INSERM European Unit (LEA) (INSERM, Paris, France), University of Fribourg, Fribourg, Switzerland
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
出版信息
Antimicrob Agents Chemother. 2016 Jun 20;60(7):4394-7. doi: 10.1128/AAC.00444-16. Print 2016 Jul.
Abstract
A series of colistin-resistant Escherichia coli clinical isolates was recovered from hospitalized and community patients in South Africa. Seven clonally unrelated isolates harbored the mcr-1 gene located on different plasmid backbones. Two distinct plasmids were fully sequenced, and identical 2,600-bp-long DNA sequences defining a mcr-1 cassette were identified. Promoter sequences responsible for the expression of mcr-1, deduced from the precise identification of the +1 transcription start site for mcr-1, were characterized.
摘要
从南非的住院患者和社区患者中分离出一系列耐黏菌素的大肠杆菌临床菌株。七株克隆无关的菌株携带位于不同质粒骨架上的mcr-1基因。对两个不同的质粒进行了全序列测定，并鉴定出定义mcr-1盒的相同的2600bp长的DNA序列。通过精确鉴定mcr-1的+1转录起始位点推导得出负责mcr-1表达的启动子序列，并对其进行了表征。
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