Carvalho Karine da Silva, E Silva Sandra Lúcia da Cunha, de Souza Ivone Antonia, Gualberto Simone Andrade, da Cruz Rômulo Carlos Dantas, Dos Santos Frances Regiane, de Carvalho Mário Geraldo
Mestranda em Ciências Ambientais - Programa de Pós-Graduação em Ciências Ambientais (PPGCA), Universidade Estadual do Sudoeste da Bahia (UESB), Itapetinga, BA, Brazil.
Núcleo de Pesquisa em Química Aplicada/UESB, Praça Primavera, 40, Bairro Primavera, 45700-000, Itapetinga, BA, Brazil.
Parasitol Res. 2016 Sep;115(9):3441-8. doi: 10.1007/s00436-016-5106-2. Epub 2016 May 12.
For control of Aedes aegypti, the main vector of dengue, botanical insecticides can be a viable alternative. Herein, we evaluated the chemical composition and insecticidal activity of the essential oils of the leaves of Croton tetradenius on Ae. aegypti larvae and adults. We also evaluated the acute toxicity in Mus musculus. The essential oil chemical analysis was performed using chromatography coupled with mass spectrometry and flame ionization detection. Female mice were used for assessing toxicity according to the Organization for Economic Cooperation and Development's Test Guideline 423/2001. Doses administered to mice orally and intraperitoneally were 5, 50, 300, and 2000 mg kg(-1). There was a greater toxic effect on larvae (LC50 = 0.152 mg mL(-1) and LC90 = 0.297 mg mL(-1)) and on adults (LC50 = 1.842 mg mL(-1) and LC90 = 3.156 mg mL(-1)) of Ae. aegypti after 24 h of exposure, when compared to other periods of exposure. Chemical analysis revealed 26 components, with camphor (25.49 %) as the major component. The acute toxicity via the intraperitoneal route identified an LD50 = 200 mg kg(-1) and by the oral route an LD50 = 500 mg kg(-1). Thus, the essential oil of C. tetradenius presents insecticidal potential for Ae. aegypti and has high safety threshold at the concentrations evaluated in this study.
对于登革热的主要传播媒介埃及伊蚊的防治，植物源杀虫剂可能是一种可行的替代方法。在此，我们评估了巴豆叶精油对埃及伊蚊幼虫和成虫的化学成分及杀虫活性。我们还评估了其对小家鼠的急性毒性。采用色谱-质谱联用和火焰离子化检测对精油进行化学分析。根据经济合作与发展组织的测试指南423/2001，使用雌性小鼠评估毒性。经口和腹腔注射给予小鼠的剂量分别为5、50、300和2000 mg kg⁻¹。与其他暴露时间段相比，暴露24小时后对埃及伊蚊幼虫（LC50 = 0.152 mg mL⁻¹，LC90 = 0.297 mg mL⁻¹）和成虫（LC50 = 1.842 mg mL⁻¹，LC90 = 3.156 mg mL⁻¹）的毒性作用更大。化学分析显示有26种成分，其中樟脑（25.49%）为主要成分。腹腔注射途径的急性毒性确定LD50 = 200 mg kg⁻¹，经口途径LD50 = 500 mg kg⁻¹。因此，巴豆叶精油对埃及伊蚊具有杀虫潜力，并且在本研究评估的浓度下具有较高的安全阈值。
