基于下一代测序技术的莼菜（睡莲科）微卫星位点的开发与特征分析
Development and characterization of microsatellite loci in Brasenia schreberi (Cabombaceae) based on the next-generation sequencing.
作者信息
Liu H, Long Z C, Li L N, Wang Q F, Chen J M, Liao K
机构信息
College of Life Science, South-Central University for Nationalities, Wuhan, Hubei, China.
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.
出版信息
Genet Mol Res. 2016 Apr 4;15(2):gmr7886. doi: 10.4238/gmr.15027886.
To understand genetic variability of the endangered aquatic herb Brasenia schreberi (Cabombaceae), we describe 31 microsatellite markers obtained using next-generation sequencing. A total of 24 individuals from the population of Jackson Lake, USA, were genotyped for each marker. Twenty-eight markers were polymorphic. The number of alleles per locus ranged from 1 to 9; the observed and expected heterozygosities ranged from 0 to 1 and from 0 to 0.751, respectively. These markers should be useful tools for genetic variation and conservation studies of B. schreberi.
为了解濒危水生草本植物莼菜（Cabombaceae）的遗传变异性，我们描述了通过下一代测序获得的31个微卫星标记。对来自美国杰克逊湖种群的总共24个个体进行了每个标记的基因分型。28个标记是多态性的。每个位点的等位基因数量从1到9不等；观察到的杂合度和预期杂合度分别从0到1和从0到0.751不等。这些标记应该是莼菜遗传变异和保护研究的有用工具。
Zotero 插件