Trudel M, Nadon F, Séguin C, Simard C, Lussier G
Centre de Recherche en Virologie, Institut Armand-Frappier, Université du Québec, Ville de Laval, Canada.
Vaccine. 1989 Feb;7(1):12-6. doi: 10.1016/0264-410x(89)90004-2.
The purpose of the present study was to evaluate experimentally, in guinea-pigs, the immunogenicity of respiratory syncytial (RS) virus subunit vaccines. Immunostimulating complexes (ISCOMs), made from the surface proteins of both human (Long) and bovine (A-51908) RS strains adsorbed to the adjuvant Quil A, were assayed for their capacity to induce neutralizing antibodies, in comparison to experimental live virus vaccines. Serums from animals vaccinated with either the human or bovine RS subunit vaccines were equally efficient in neutralizing human or bovine RS virus. ISCOMs prepared with bovine RS virus proteins were significantly (p less than 0.05%) more efficient than their human counterpart, in inducing neutralizing antibodies, suggesting their greater potential as a subunit vaccine.
本研究的目的是在豚鼠身上通过实验评估呼吸道合胞（RS）病毒亚单位疫苗的免疫原性。将来自人（Long株）和牛（A-51908株）RS病毒株表面蛋白吸附于佐剂Quil A制成的免疫刺激复合物（ISCOMs），与实验性活病毒疫苗相比，检测其诱导中和抗体的能力。接种人源或牛源RS亚单位疫苗的动物血清在中和人源或牛源RS病毒方面同样有效。用牛RS病毒蛋白制备的ISCOMs在诱导中和抗体方面比用人RS病毒蛋白制备的ISCOMs显著更有效（p小于0.05%），表明其作为亚单位疫苗具有更大的潜力。
