Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Egypt.
Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Egypt.
J Adv Res. 2016 May;7(3):413-21. doi: 10.1016/j.jare.2016.02.008. Epub 2016 Mar 5.
To study the impact of radish oil on the possible genotoxic and hepatotoxic effects of hexavalent chromium, male rats were divided into 4 groups. Group 1 served as control, group 2 received radish oil at the recommended human therapeutic dose (0.07 mL/kg) by gavage, group 3 received sodium dichromate dihydrate (SDD) 520 mg/L in drinking water, and group 4 received both SDD and radish oil as previously mentioned in groups 2 and 3. All treatments were continued for six months. The results revealed that chromium exposure promoted oxidative stress with a consequently marked hepatic histopathological alterations, increased serum alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities, alfa fetoprotein (AFP) levels, and micronucleated erythrocytes (MNE) % in peripheral blood. Moreover, COMET assay of hepatic DNA revealed that SDD exposure significantly decreased the intact cells %, head diameter, and head DNA % compared to control, indicating DNA damage. However, radish oil co-administration with SDD resulted in marked amendment in the altered parameters as detected by improved liver function markers (ALT and ALP) and AFP level, decreased lipid peroxidation, increased antioxidant markers, inhibited hepatic DNA damage and restored the hepatic histology by preventing the appearance of the altered hepatocytes' foci and decreasing chromium induced histopathological lesions. It could be concluded that radish oil was able to provide a convergent complete protection against the geno- and hepatotoxicity of chromium by its potent antioxidant effect.
为了研究萝卜油对六价铬可能产生的遗传毒性和肝毒性的影响，雄性大鼠被分为 4 组。第 1 组为对照组，第 2 组以推荐的人类治疗剂量（0.07ml/kg）经灌胃给予萝卜油，第 3 组给予二水合重铬酸钠（SDD）520mg/L 的饮用水，第 4 组给予 SDD 和萝卜油，剂量同第 2 组和第 3 组。所有处理均持续 6 个月。结果表明，铬暴露会促进氧化应激，导致明显的肝组织病理学改变，增加血清丙氨酸氨基转移酶（ALT）和碱性磷酸酶（ALP）活性、α-胎蛋白（AFP）水平和外周血微核红细胞（MNE）%。此外，肝 DNA 的 COMET 分析表明，与对照组相比，SDD 暴露显著降低了完整细胞%、头部直径和头部 DNA%，表明存在 DNA 损伤。然而，萝卜油与 SDD 联合给药可显著改善 ALT 和 ALP 等改变参数以及 AFP 水平，降低脂质过氧化，增加抗氧化标志物，抑制肝 DNA 损伤，并通过防止出现改变的肝细胞灶和减少铬诱导的组织病理学损伤，恢复肝组织学。可以得出结论，萝卜油通过其强大的抗氧化作用，能够对铬的遗传毒性和肝毒性提供全面的保护作用。
