BACKGROUND: Post-acne facial scarring has always been a challenge to treat. It requires multiple therapeutic modalities as single modality is not hundred percent effective. Therefore, we have combined CO laser resurfacing with subcision in patients with acne scars for better results. OBJECTIVES: The aim is to study the effectiveness and side effects of CO laser with subcision in patients with atrophic acne scars. METHODS: Fifty patients were selected for the study. Baseline grading was done with Goodman and Baron grading system. Twenty-five patients were randomly selected for subcision followed by CO laser and the remaining patients were selected for CO laser alone. The treatment was done for four sessions at 4-week interval. Clinical photographs were obtained for evaluation. RESULTS: CO laser with subcision showed excellent response in grade-2 and -3 acne scars. Statistically there is a significant difference between CO laser following subcision and CO laser alone at 5% level (p < 0.05). Both procedures were well tolerated with minimal side effects. CONCLUSION: The highly versatile CO laser is useful for treating acne scars. Subcision prior to the CO laser procedure showed better improvement when compared to CO laser alone. Thus, in acne scars, multiple therapeutic modalities achieve better results.