超声引导下光声成像用于选择性检测表皮生长因子受体（EGFR）表达的乳腺癌及淋巴结转移灶。

Ultrasound-guided photoacoustic imaging for the selective detection of EGFR-expressing breast cancer and lymph node metastases.

作者信息

Zhang Meihua, Kim Hoe Suk, Jin Tiefeng, Yi Ann, Moon Woo Kyung

机构信息

Department of Science and Radiology, College of Medicine, Seoul National University, 103 Daehak-ro, Jongno-gu, Seoul 03080, South Korea; Contributed equally.

Department of Radiology, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, South Korea; Contributed equally.

出版信息

Biomed Opt Express. 2016 Apr 19;7(5):1920-31. doi: 10.1364/BOE.7.001920. eCollection 2016 May 1.

DOI:10.1364/BOE.7.001920

PMID:27231631

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4871091/

Abstract

We assessed the use of ultrasound (US)-guided photoacoustic imaging (PAI) and anti-EGFR antibody-conjugated gold nanorods (anti-EGFR-GNs) to non-invasively detect EGFR-expressing primary tumor masses and regional lymph node (LN) metastases in breast tumor mice generated by injecting MCF-7 (EGFR-negative) or MDA-MB-231 (EGFR-positive) human breast cells using a preclinical Vevo 2100 LAZR Imaging system. Anti-EGFR-GNs provided a significant enhancement in the PA signal in MDA-MB-231 tumor and the axillary LN metastases relative to MCF-7 tumor and non-LN metastases. We demonstrated that US-guided PAI using anti-EGFR-GNs is highly sensitive for the selective visualization of EGFR-expressing breast primary tumors as well as LN micrometastases.

摘要

我们使用临床前Vevo 2100 LAZR成像系统，评估了超声（US）引导的光声成像（PAI）和抗表皮生长因子受体（EGFR）抗体偶联金纳米棒（抗EGFR-GNs）在非侵入性检测注射MCF-7（EGFR阴性）或MDA-MB-231（EGFR阳性）人乳腺癌细胞所产生的乳腺肿瘤小鼠中表达EGFR的原发性肿瘤肿块和区域淋巴结（LN）转移方面的应用。相对于MCF-7肿瘤和非LN转移，抗EGFR-GNs使MDA-MB-231肿瘤和腋窝LN转移中的PA信号显著增强。我们证明，使用抗EGFR-GNs的US引导PAI对表达EGFR的乳腺原发性肿瘤以及LN微转移的选择性可视化具有高度敏感性。