Key Lab of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Lab for Marine Science and Technology. Qingdao 266071, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
Key Lab of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Lab for Marine Science and Technology. Qingdao 266071, China; State Key Lab of Seaweed Bioactive Substances, Qingdao 266000, China.
Int J Biol Macromol. 2016 Oct;91:233-40. doi: 10.1016/j.ijbiomac.2016.05.081. Epub 2016 May 24.
Diabetic nephropathy (DN) is a serious microvascular complication that can lead to chronic and end-stage renal failure. It is understood that inflammation is associated with the onset and process of DN. Low molecular weight fucoidan (LMWF) isolated from Saccharina japonica has anti-inflammatory properties. Therefore, this study aimed to explore the mechanism of LMWF in DN model induced by streptozotocin. The biochemical indices levels showed LMWF reduced the DN diagnostic indices to protect renal function. The HE stained sections exhibited LMWF protected normal morphological structures and reduced inflammatory cell infiltration in the kidneys of DN rats. Furthermore, the levels of P-selectin and selectin-dependent inflammatory cytokines resulting from LMWF were obviously decreased at both the transcriptional and protein levels. Thus, our results found that LMWF protected the renal function in DN rats and alleviated inflammation through the modulation of P-selectin and inflammatory cytokines. LMWF may have therapeutic potential against DN.
糖尿病肾病（DN）是一种严重的微血管并发症，可导致慢性和终末期肾衰竭。据了解，炎症与 DN 的发病和进程有关。从裙带菜中分离得到的低分子量褐藻糖胶（LMWF）具有抗炎作用。因此，本研究旨在探讨 LMWF 在链脲佐菌素诱导的 DN 模型中的作用机制。生化指标水平显示，LMWF 降低了 DN 的诊断指标，从而保护了肾功能。HE 染色切片显示，LMWF 保护了 DN 大鼠肾脏的正常形态结构，减少了炎症细胞浸润。此外，LMWF 使 P-选择素和选择素依赖性炎症细胞因子的水平在转录和蛋白水平上明显降低。因此，我们的研究结果发现，LMWF 通过调节 P-选择素和炎症细胞因子来保护 DN 大鼠的肾功能并减轻炎症。LMWF 可能具有治疗 DN 的潜力。
