Rahman Md Arifur, Alam Md Mahbub, Islam Md Aminul, Bhuiyan A K Fazlul Haque, Rahman A K M Anisur
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh 2202, Bangladesh.
Department of Animal Breeding and Genetics, Faculty of Animal Husbandry, BAU, Mymensingh 2202, Bangladesh.
Vet Med Int. 2016;2016:9098416. doi: 10.1155/2016/9098416. Epub 2016 Apr 30.
The objective of this study was to know the herd and animal level prevalence of Q fever in domestic ruminants in some selected districts in Bangladesh. Randomly collected 111 bulk milk and 94 sera samples of cattle, sheep, and goats were tested by indirect ELISA (iELISA). DNA extracted from 23 aborted fetal membranes was analyzed by real time (rt) PCR. The positive cut-off value of iELISA in bulk milk and individual animal sera was ≥30% and ≥40%, respectively. The overall herd level prevalence of Q fever in dairy cattle was 15.6%. The prevalence of Q fever in dairy cattle was significantly higher in Sirajganj in comparison to Satkhira District (P < 0.01). The overall seroprevalence of Q fever in domestic ruminants was 5.06%. Although statistically insignificant, the seroprevalence of Q fever was relatively higher in sheep (9.52%) in comparison to goats (3.33%) and cattle (3.57%). Out of 23 aborted fetal membranes, only one sheep placenta was positive in rt PCR. Q fever is present in all of the three important species of domestic ruminants in Bangladesh. It may have some role in sheep abortion as the seroprevalence is relatively higher and also one sheep placenta is rt PCR positive.
本研究的目的是了解孟加拉国一些选定地区家养反刍动物群体和个体层面的Q热流行情况。通过间接酶联免疫吸附测定法（iELISA）对随机采集的111份牛、羊和山羊的混合乳样以及94份血清样本进行检测。对从23份流产胎膜中提取的DNA进行实时（rt）聚合酶链反应分析。iELISA在混合乳和个体动物血清中的阳性临界值分别为≥30%和≥40%。奶牛群体层面Q热的总体流行率为15.6%。与萨特希拉区相比，锡拉杰甘杰的奶牛Q热流行率显著更高（P < 0.01）。家养反刍动物Q热的总体血清阳性率为5.06%。虽然无统计学意义，但与山羊（3.33%）和牛（3.57%）相比，绵羊的Q热血清阳性率相对较高（9.52%）。在23份流产胎膜中，rt PCR检测只有一份绵羊胎盘呈阳性。孟加拉国所有三种重要的家养反刍动物均存在Q热。由于血清阳性率相对较高且一份绵羊胎盘rt PCR呈阳性，它可能在绵羊流产中起一定作用。
