Özel Demiralp Duygu, Haznedaroglu İbrahim C, Akar Nejat
Proteomics Unit, Ankara University Biotechnology Institute, 06100 Ankara, Turkey Phone: +90 312 222 58 17 - +90 312 222 58 26 E-mail:
Turk J Haematol. 2010 Jun 5;27(2):70-7. doi: 10.5152/tjh.2010.03.
Ankaferd® Blood Stopper (ABS) comprises a standardized mixture of the plants Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum, and Urtica dioica. The basic mechanism of action for ABS is the formation of an encapsulated protein network that provides focal points for vital erythrocyte aggregation. ABS-induced protein network formation with blood cells, particularly erythrocytes, covers the primary and secondary hemostatic system without disturbing individual coagulation factors.
To understand the effect mechanisms of ABS on hemostasis, a proteomic analysis using 2D gel electrophoresis and mass spectrometer was performed.
Proteins of plant origin in Ankaferd® were NADP-dependent-malic enzyme, ribulose bisphosphate-carboxylase-large chain, maturase K, ATP synthase subunit-beta, ATP synthase subunit-alpha, chalcone-flavanone isomerase-1, chalcone-flavanone isomerase-2, and actin-depolymerizing factor. Furthermore, functional proteomic studies revealed that proteins resembling human peptides have been detected within Ankaferd®, including ATP synthase, mucin-16 (CD164 sialomucin-like 2 protein), coiled-coil domain containing 141 hypothetical protein LOC283638 isoform 1, hypothetical protein LOC283638 isoform 2, dynactin 5, complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, TP synthase, H+ transporting, mitochondrial actin binding 1 isoform, LIM domain and actin binding 1 isoform a, LIM domain and actin binding 1 isoform b, spectrin alpha non erythrocytic 1, prolactin releasing hormone receptor, utrophin, tet oncogene family member 2 isoform b, protein phosphatase 1 regulatory subunit 12A, NIMA (never in mitosis gene a)-related kinase, ATP-binding cassette protein C12, Homo sapiens malic enzyme 1, mitochondrial NADP(+)-dependent malic enzyme 3, ME2 protein, nuclear factor 1 B-type, abhydrolase domain-containing protein 12B, E3 SUMO-protein ligase PIAS2, alpha-1, 2-glucosyltransferase ALG10-A, cofilin, non-muscle isoform, 18 kDa phosphoprotein, p18, actin-depolymerizing factor (ADF), twinfilin-1, ankyrin repeat and FYVE domain-containing protein 1, usherin precursor, urotensin II receptor, interleukin 4, and midkine.
Proteomic analysis of Ankaferd® represents a true basis for the upcoming Ankaferd® studies focusing on its wound healing, hemostatic, anti-infective, antineoplastic, and preservative biological actions.
安卡菲德血液止血剂（ABS）由百里香、光果甘草、葡萄、高良姜和异株荨麻的标准化植物混合物组成。ABS的基本作用机制是形成一个包裹性的蛋白质网络，为重要的红细胞聚集提供聚集点。ABS诱导的蛋白质网络与血细胞（尤其是红细胞）形成，覆盖初级和次级止血系统，而不干扰单个凝血因子。
为了解ABS对止血的作用机制，采用二维凝胶电泳和质谱仪进行蛋白质组学分析。
安卡菲德中的植物源性蛋白质有依赖NADP的苹果酸酶、1,5-二磷酸核酮糖羧化酶大亚基、成熟酶K、ATP合酶β亚基、ATP合酶α亚基、查尔酮-黄烷酮异构酶-1、查尔酮-黄烷酮异构酶-2和肌动蛋白解聚因子。此外，功能蛋白质组学研究表明，在安卡菲德中检测到了类似人类肽的蛋白质，包括ATP合酶、粘蛋白-16（CD164唾液酸粘蛋白样2蛋白）、含卷曲螺旋结构域的141号假设蛋白LOC283638亚型1、假设蛋白LOC283638亚型2、动力蛋白5、复合物I中间相关蛋白30、线粒体、NADH脱氢酶（泛醌）1α亚复合物、TP合酶、H⁺转运、线粒体肌动蛋白结合1亚型、LIM结构域和肌动蛋白结合1亚型a、LIM结构域和肌动蛋白结合1亚型b、非红细胞血影蛋白α、催乳素释放激素受体、肌养蛋白、tet癌基因家族成员2亚型b、蛋白磷酸酶1调节亚基12A、NIMA（从不处于有丝分裂基因a）相关激酶、ATP结合盒蛋白C12、智人苹果酸酶1、线粒体NADP⁺依赖性苹果酸酶3、ME2蛋白、核因子1 B型、含水解酶结构域蛋白12B、E3 SUMO蛋白连接酶PIAS2、α-1,2-葡糖基转移酶ALG10-A、丝切蛋白、非肌肉亚型、18 kDa磷蛋白、p18、肌动蛋白解聚因子（ADF）、双丝蛋白-1、含锚蛋白重复序列和FYVE结构域蛋白1、前庭膜蛋白前体、尾加压素II受体、白细胞介素4和中期因子。
安卡菲德的蛋白质组学分析是即将开展的关于其伤口愈合、止血、抗感染、抗肿瘤和防腐生物学作用的安卡菲德研究的真正基础。
