Department of Chemical Engineering, ‡Department of Macromolecular Science & Engineering, §Department of Material Science & Engineering, ⊥Department of Biomedical Engineering, and ∥Biointerfaces Institute, University of Michigan , Ann Arbor, Michigan 48109, United States.
ACS Appl Mater Interfaces. 2016 Jul 6;8(26):16595-603. doi: 10.1021/acsami.6b04370. Epub 2016 Jun 21.
The performance of polymer interfaces in biology is governed by a wide spectrum of interfacial properties. With the ultimate goal of identifying design parameters for stem cell culture coatings, we developed a statistical model that describes the dependence of brush properties on surface-initiated polymerization (SIP) parameters. Employing a design of experiments (DOE) approach, we identified operating boundaries within which four gel architecture regimes can be realized, including a new regime of associated brushes in thin films. Our statistical model can accurately predict the brush thickness and the degree of intermolecular association of poly[{2-(methacryloyloxy) ethyl} dimethyl-(3-sulfopropyl) ammonium hydroxide] (PMEDSAH), a previously reported synthetic substrate for feeder-free and xeno-free culture of human embryonic stem cells. DOE-based multifunctional predictions offer a powerful quantitative framework for designing polymer interfaces. For example, model predictions can be used to decrease the critical thickness at which the wettability transition occurs by simply increasing the catalyst quantity from 1 to 3 mol %.
聚合物界面在生物学中的性能受广泛的界面性质控制。为了确定干细胞培养涂层的设计参数，我们开发了一个统计模型，描述了刷特性对表面引发聚合（SIP）参数的依赖关系。我们采用实验设计（DOE）方法，确定了可以实现四个凝胶结构区域的操作边界，包括在薄膜中形成新的缔合刷的区域。我们的统计模型可以准确预测聚[{2-（甲基丙烯酰氧基）乙基}二甲（3-磺丙基）铵氢氧化物]（PMEDSAH）的刷厚度和分子间缔合程度，PMEDSAH 是一种先前报道的用于无饲养细胞和无动物来源培养人类胚胎干细胞的合成底物。基于 DOE 的多功能预测为设计聚合物界面提供了强大的定量框架。例如，可以通过简单地将催化剂数量从 1 mol%增加到 3 mol%来降低润湿性转变发生的临界厚度。
