Kownacki E, Flury F
Pharm Acta Helv. 1989;64(3):68-70.
Studies have been carried out in connection with the sterilization of glass infusion bottles from which air had not been evacuated and containing certain limited quantities of water. Bottles with a capacity of 500 and 1000 cm3 were filled with 1-3 cm3 distilled water and sterilized in an autoclave for 20 min. set at a temperature of 121 degrees C reaching up to 126 degrees C. The sterility was tested by introducing bags containing Bacillus stearothermophilus spores into the centre of the bottles. Following sterilization the bottles were examined for the growth of spores allowing an incubation period of 7 days at 37 degrees C. Based on the actual conditions of this investigation, it would appear that a bottle from which air has not been evacuated should contain at least 2% (V/V) distilled water, when sterilized in an autoclave. It is, however, strongly recommended that the present findings should be validated based on actual conditions prevailing for individual sterilization procedures.
针对未抽真空且含有一定限量水的玻璃输液瓶的灭菌进行了研究。容量为500立方厘米和1000立方厘米的瓶子装入1至3立方厘米蒸馏水，在高压灭菌器中于设定温度121摄氏度、最高达126摄氏度下灭菌20分钟。通过将装有嗜热脂肪芽孢杆菌孢子的袋子放入瓶子中央来测试无菌性。灭菌后，将瓶子在37摄氏度下培养7天，检查是否有孢子生长。基于本次调查的实际情况，似乎未抽真空的瓶子在高压灭菌器中灭菌时应至少含有2%（V/V）的蒸馏水。然而，强烈建议根据各个灭菌程序的实际情况对目前的研究结果进行验证。
