Liu Wenhua, Han Letian, Song Peng, Sun Huzhi, Zhang Can, Zou Ling, Cui Jiaqi, Pan Qiang, Ren Huiying
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
Qingdao Phagepharm Bio-tech Co., Ltd., Qingdao, Shandong, China.
Front Microbiol. 2022 Aug 16;13:938616. doi: 10.3389/fmicb.2022.938616. eCollection 2022.
Salmonella enterica subspecies enterica serovar abortus equi () is the most common cause of abortion in mares. It has recently been found to cause abortion in donkeys more frequently in China. A novel virulent bacteriophage vB_SabS_Sds2 (hereafter designated as Sds2) was isolated from the feces of donkeys using a strain as a host. Phage Sds2 had an isometric polyhedral head and an uncontracted long tail, belonging to the , , , . The genome of phage Sds2 was 114,770 bp, with a GC content of 40.26%. The genome contained 160 open reading frames (ORFs), and no ORFs were associated with pathogenicity, drug resistance, or lysogenization by sequence analysis. Both genome annotation and phylogenetic analysis indicated that phage Sds2 was highly similar to T5-like bacteriophages. Phage Sds2 could lyse 100% (30/30) of strains, 25.3% (24/95) of other serotypes of strains, and 27.6% (8/29) of strains using the double-layer agar plate method. The test showed that phage Sds2 had high bactericidal activity against at a wide range of MOIs. The test indicated that phage Sds2 had an inhibitory effect on abortion in mice challenged with In general, phage Sds2 is a novel lytic phage with a wide host range and has the potential to prevent abortion caused by
肠炎沙门氏菌亚种肠炎血清型马流产沙门氏菌（Salmonella enterica subspecies enterica serovar abortus equi）是母马流产最常见的原因。最近在中国发现它在驴中导致流产的情况更为频繁。使用一株菌株作为宿主，从驴粪便中分离出一种新型烈性噬菌体vB_SabS_Sds2（以下简称Sds2）。噬菌体Sds2具有等轴多面体头部和未收缩的长尾，属于[具体类别未给出]。噬菌体Sds2的基因组为114,770 bp，GC含量为40.26%。该基因组包含160个开放阅读框（ORF），通过序列分析没有发现与致病性、耐药性或溶原化相关的ORF。基因组注释和系统发育分析均表明噬菌体Sds2与T5样噬菌体高度相似。使用双层琼脂平板法，噬菌体Sds2能裂解100%（30/30）的[菌株名称未给出]菌株、25.3%（24/95）的其他血清型[菌株名称未给出]菌株以及27.6%（8/29）的[菌株名称未给出]菌株。一步生长试验表明噬菌体Sds2在广泛的感染复数（MOI）范围内对[菌株名称未给出]具有高杀菌活性。动物实验表明噬菌体Sds2对感染[菌株名称未给出]的小鼠流产具有抑制作用。总体而言，噬菌体Sds2是一种具有广泛宿主范围的新型裂解性噬菌体，具有预防由[菌株名称未给出]引起的流产的潜力
